Rahimi Z, Yari K, Rahimi Z
Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran.
Kermanshah University of Medical Sciences Medical Biology Research Center Kermanshah Iran
Cell Mol Biol (Noisy-le-grand). 2016 Feb 4;62(2):21-4.
Matrix metalloproteinases (MMPs) are involved in multiple physiological and pathological processes. Variable frequency of the MMPs gene variants might affect the susceptibility to certain diseases. The aim of present study was to investigate the frequency of MMP-7 A-181G and MMP-2 C-735T variants in healthy population of Western Iran with Kurdish ethnic background. Individuals were medical students and staff members of the Medical School of Kermanshah University and blood donors that consisted of 221 females and 94 males. Control subjects were free of general and genetic diseases. Two hundred and eighty available samples including 192 females and 88 males were studied for MMP-2 C-735T polymorphism. Genomic DNA was extracted from peripheral blood leukocytes. The MMP-7 A-181G and MMP-2 C-735T polymorphisms were detected using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The prevalence of MMP-7 G allele was 40% in studied individuals. The overall frequency of MMP-2 -735T allele was 15%. There was a higher frequency of MMP-2 T allele in females (16.9%) compared to males (10.8%, p=0.059). There were 30 (13.6%) women and 8 men (8.5%) with concomitant presence of MMP-7 AG and MMP-2 CT genotypes. All nine (4.1%) individuals with combined presence of MMP-7 GG and MMP-2 CT genotypes were women. The present study reports the frequency of two MMPs gene polymorphisms in healthy population of Western Iran. Our findings might be useful in evaluating the risk of MMPs in certain diseases. Also, our study suggests genetic admixture and similarities between our population with some Asian and European populations.
基质金属蛋白酶（MMPs）参与多种生理和病理过程。MMPs基因变体的频率变化可能会影响对某些疾病的易感性。本研究的目的是调查伊朗西部库尔德族裔健康人群中MMP-7 A-181G和MMP-2 C-735T变体的频率。研究对象为克尔曼沙赫大学医学院的医学生、工作人员和献血者，其中包括221名女性和94名男性。对照受试者无全身性疾病和遗传性疾病。对280份可用样本（包括192名女性和88名男性）进行了MMP-2 C-735T多态性研究。从外周血白细胞中提取基因组DNA。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测MMP-7 A-181G和MMP-2 C-735T多态性。在研究对象中，MMP-7 G等位基因的患病率为40%。MMP-2 -735T等位基因的总体频率为15%。与男性（10.8%，p=0.059）相比，女性中MMP-2 T等位基因的频率更高（16.9%）。同时存在MMP-7 AG和MMP-2 CT基因型的女性有30名（13.6%），男性有8名（8.5%）。同时存在MMP-7 GG和MMP-2 CT基因型的所有9名（4.1%）个体均为女性。本研究报告了伊朗西部健康人群中两种MMPs基因多态性的频率。我们的研究结果可能有助于评估某些疾病中MMPs的风险。此外，我们的研究表明我们的人群与一些亚洲和欧洲人群之间存在基因混合和相似性。
