Saremi Leila, Esmaeili Saharnaz, Ghaffari Mohammad Ebrahim, Shahbazi Soheil, Lotfipanah Shirin, Kadkhodazadeh Mahdi
Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Clin Oral Investig. 2023 Dec;27(12):7417-7423. doi: 10.1007/s00784-023-05331-5. Epub 2023 Oct 17.
The current study aimed to investigate the association of matrix metalloproteinase- (MMP-) 1, -2, -3, -7, and -13 gene polymorphisms with chronic periodontitis (CP) in an Iranian population.
In this case-control study, 87 subjects with CP and 89 periodontally healthy subjects were allocated to case and control groups, respectively. Subjects' venous blood samples (5 cc) were collected, and DNA extraction was performed. A spectrophotometer was utilized to assess the concentration of extracted DNAs. The desired gene polymorphisms were examined using restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) followed by electrophoresis. Statistical analyses were done using the Pearson Chi-Square test, odds ratio, and t-Test using SPSS Version 28.
The MMP-1 (-1607 1G/2G) rs1799750, MMP-3 (-1171 5A/6A) rs3025058, and MMP-7 (-181 A/G) rs11568818 gene polymorphisms significantly differed between case and control groups (PV = 0.019, 0.007, and 0.028, respectively). In contrast, the gene polymorphisms of MMP-2 (-1306 C/T) rs243865 and MMP-13 (-77 A/G) rs2252070 did not make a significant difference. Regarding allele frequencies, the presence of the 2G allele in the MMP-1 (-1607) rs1799750 genotype increased the CP susceptibility significantly, while subjects with the 6A allele in their MMP-3 (-1171) rs3025058 genotype showed significantly lower susceptibility to CP (PV = 0.008 and < 0.001, respectively).
In the studied population, gene polymorphisms in the DNA sequences of MMP-1 (-1607 1G/2G) rs1799750, MMP-3 (-1171 5A/6A) rs3025058, and MMP-7 (-181 A/G) rs11568818 may have impacts on CP incidence.
Clinicians should be cautious about the association between MMP-1, MMP-3, and MMP-7 gene polymorphisms and the incidence of chronic periodontitis during periodontal treatment planning.
本研究旨在调查伊朗人群中基质金属蛋白酶（MMP）-1、-2、-3、-7和-13基因多态性与慢性牙周炎（CP）的相关性。
在这项病例对照研究中，87例慢性牙周炎患者和89例牙周健康受试者分别被分配到病例组和对照组。采集受试者的静脉血样本（5毫升）并进行DNA提取。使用分光光度计评估提取的DNA浓度。采用限制性片段长度多态性聚合酶链反应（RFLP-PCR）及随后的电泳检测所需的基因多态性。使用SPSS 28版软件进行Pearson卡方检验、比值比和t检验进行统计分析。
病例组和对照组之间MMP-1（-1607 1G/2G）rs1799750、MMP-3（-1171 5A/6A）rs3025058和MMP-7（-181 A/G）rs11568818基因多态性存在显著差异（P值分别为0.019、0.007和0.028）。相比之下，MMP-2（-1306 C/T）rs243865和MMP-13（-77 A/G）rs2252070的基因多态性无显著差异。关于等位基因频率，MMP-1（-1607）rs1799750基因型中2G等位基因的存在显著增加了患慢性牙周炎的易感性，而MMP-3（-1171）rs3025058基因型中6A等位基因的受试者患慢性牙周炎的易感性显著降低（P值分别为0.008和<0.001）。
在所研究的人群中，MMP-1（-1607 1G/2G）rs1799750、MMP-3（-1171 5A/6A）rs3025058和MMP-7（-181 A/G）rs11568818基因序列中的基因多态性可能对慢性牙周炎的发病率有影响。
临床医生在进行牙周治疗计划时，应谨慎考虑MMP-1、MMP-3和MMP-7基因多态性与慢性牙周炎发病率之间的关联。
