Carrizo Daniel, Nerín Isabel, Domeño Celia, Alfaro Pilar, Nerín Cristina
Aragon Institute of Engineering Research (I3A), EINA, Department of Analytical Chemistry, University of Zaragoza, María de Luna 3, 50018 Zaragoza, Spain.
Smoking Cessation Unit, Department of Medicine, Psychiatry and Dermatology, Faculty of Medicine, University of Zaragoza, Domingo Miral s/n, 50009 Zaragoza, Spain.
J Pharm Biomed Anal. 2016 May 30;124:149-156. doi: 10.1016/j.jpba.2016.02.033. Epub 2016 Feb 26.
A new screening method has been explored for direct analysis of tobacco smoke biomarkers in biological matrices (i.e., saliva and urine). Single run analysis using Atmospheric pressure Solid Analysis Probe (ASAP) and high resolution mass spectrometry with quadrupole and time of flight detector has been applied directly to some biological samples (i.e., urine and saliva), providing a fast, efficient and sensitive method of identification. The method has been applied to saliva and urine samples from heavy tobacco smokers for exposure studies. Nicotine itself, nicotine metabolites (i.e., cotinine, trans-3'-hydroxycotinine, nicotine-N-glucuronide) and other related tobacco smoke toxic compounds (i.e., NNK 4-[methyl(nitroso)amino]-1-(3-pyridinyl)-1-butanone, anatabine) were found in the analyzed samples. The identification of compounds was confirmed by ultrahigh performance liquid chromatography with MS-triple quadrupole detector after sample treatment. Different temporal trends and biomarkers behavior have been found in time series related samples. Both methods are compared for screening of these biological matrices.
已探索出一种新的筛查方法，用于直接分析生物基质（即唾液和尿液）中的烟草烟雾生物标志物。使用大气压固体分析探头（ASAP）以及带有四极杆和飞行时间检测器的高分辨率质谱进行的单次运行分析已直接应用于一些生物样品（即尿液和唾液），提供了一种快速、高效且灵敏的鉴定方法。该方法已应用于重度吸烟者的唾液和尿液样本以进行暴露研究。在所分析的样本中发现了尼古丁本身、尼古丁代谢物（即可替宁、反式-3'-羟基可替宁、尼古丁-N-葡萄糖醛酸）以及其他相关的烟草烟雾有毒化合物（即NNK 4-[甲基（亚硝基）氨基]-1-（3-吡啶基）-1-丁酮、去甲新烟草碱）。样品处理后，通过超高效液相色谱与质谱-三重四极杆检测器联用对化合物进行了鉴定确认。在时间序列相关样本中发现了不同的时间趋势和生物标志物行为。对这两种方法在这些生物基质的筛查方面进行了比较。
