Fluer F S, Nikolaeva I V, Pavlova T Yu, Bondarenko V M, Fialkina S V, Titarev S I
Zh Mikrobiol Epidemiol Immunobiol. 2015 Nov-Dec(6):10-7.
Determination of enterotoxigenicity and ability to synthesize TSST-1 in S. aureus strains, isolated from breast milk of women, feeding children with infectious pathology.
35 S. aureus strains, isolated from breast milk of women feeding children with varying infectious pathology in hospitals and as outpatients were studied for the presence of staphylococci enterotoxins (SE) of types A and B and toxic shock syndrome toxin (TSST-1). Determination of SEA, SEB and TSST-1 was carried out by enzyme immunoassay.
Toxins were detected in 94.2% of S. aureus strains. SEB was synthesized by 86.7%, SEA--34.3%, TSST-1--42.8% of S. aureus strains. Toxins were detected with equal frequencies in healthy women and women with inflammatory diseases of breasts. Differences in frequency of colonization of intestines of children receiving breast milk, infected with toxigenic and non-toxigenic staphylococci strains was not detected.
A high frequency of occurrence of enterotoxins and TSST-1 in S. aureus, isolated from breast milk of the mother during infectious pathology in the child was discovered. Enterotoxigenic strains can be detected in breast milk in healthy women. Study of the role of breast milk, infected with S. aureus, producing SEA, SEB And TSST-1 in development of child pathology is necessary.
测定从患有感染性疾病的哺乳期妇女母乳中分离出的金黄色葡萄球菌菌株的产肠毒素能力及合成中毒性休克综合征毒素-1（TSST-1）的能力。
对从医院及门诊中患有不同感染性疾病的哺乳期妇女母乳中分离出的35株金黄色葡萄球菌菌株，研究其是否存在A型和B型葡萄球菌肠毒素（SE）及中毒性休克综合征毒素（TSST-1）。采用酶免疫测定法测定SEA、SEB和TSST-1。
在94.2%的金黄色葡萄球菌菌株中检测到毒素。86.7%的金黄色葡萄球菌菌株合成SEB，34.3%合成SEA，42.8%合成TSST-1。在健康女性和患有乳房炎症性疾病的女性中，毒素检测频率相同。未检测到接受感染产毒和不产毒金黄色葡萄球菌菌株母乳的儿童肠道定植频率的差异。
发现从患有感染性疾病的儿童母亲母乳中分离出的金黄色葡萄球菌中产肠毒素和TSST-1的频率很高。在健康女性的母乳中也可检测到产肠毒素菌株。有必要研究感染产SEA、SEB和TSST-1的金黄色葡萄球菌的母乳在儿童疾病发生发展中的作用。
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