a Department of Laboratory Medicine , Southwest Hospital, the Third Military Medical University , Chongqing , People's Republic of China .
b Medical Contingent of the Chinese PLA to Liberia , Chongqing, People's Republic of China , and.
Crit Rev Clin Lab Sci. 2016 Oct;53(5):326-40. doi: 10.3109/10408363.2016.1160866. Epub 2016 Apr 12.
Ebola virus disease (EVD), caused by Ebola virus (EBOV), is a potent acute infectious disease with a high case-fatality rate. Etiological and serological EBOV detection methods, including techniques that involve the detection of the viral genome, virus-specific antigens and anti-virus antibodies, are standard laboratory diagnostic tests that facilitate confirmation or exclusion of EBOV infection. In addition, routine blood tests, liver and kidney function tests, electrolytes and coagulation tests and other diagnostic examinations are important for the clinical diagnosis and treatment of EVD. Because of the viral load in body fluids and secretions from EVD patients, all body fluids are highly contagious. As a result, biosafety control measures during the collection, transport and testing of clinical specimens obtained from individuals scheduled to undergo EBOV infection testing (including suspected, probable and confirmed cases) are crucial. This report has been generated following extensive work experience in the China Ebola Treatment Center (ETC) in Liberia and incorporates important information pertaining to relevant diagnostic standards, clinical significance, operational procedures, safety controls and other issues related to laboratory testing of EVD. Relevant opinions and suggestions are presented in this report to provide contextual awareness associated with the development of standards and/or guidelines related to EVD laboratory testing.
埃博拉病毒病（Ebola virus disease，EVD）由埃博拉病毒（Ebola virus，EBOV）引起，是一种具有高病死率的烈性急性传染病。埃博拉病毒的病原学和血清学检测方法，包括检测病毒基因组、病毒特异性抗原和抗病毒抗体的技术，是标准的实验室诊断检测方法，有助于确认或排除埃博拉病毒感染。此外，常规血液检查、肝肾功能检查、电解质和凝血检查等其他诊断检查对于埃博拉病毒病的临床诊断和治疗也很重要。由于埃博拉病毒病患者的体液和分泌物中的病毒载量很高，所有体液都具有高度传染性。因此，在采集、运输和检测预定进行埃博拉病毒感染检测的个体（包括疑似、可能和确诊病例）的临床标本时，必须采取生物安全控制措施。本报告是在利比里亚中国埃博拉治疗中心（China Ebola Treatment Center，ETC）的广泛工作经验基础上生成的，纳入了与相关诊断标准、临床意义、操作程序、安全控制以及与埃博拉病毒病实验室检测相关的其他问题有关的重要信息。本报告提出了相关意见和建议，旨在为制定与埃博拉病毒病实验室检测相关的标准和/或指南提供背景认识。
