通过在构巢曲霉中异源表达揭示土曲霉金黄色变种中还原型聚酮产物黄绿青霉素的生物合成途径。
Biosynthetic Pathway of the Reduced Polyketide Product Citreoviridin in Aspergillus terreus var. aureus Revealed by Heterologous Expression in Aspergillus nidulans.
作者信息
Lin Tzu-Shyang, Chiang Yi-Ming, Wang Clay C C
机构信息
Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , 1985 Zonal Avenue, Los Angeles, California 90089, United States.
Department of Pharmacy, Chia Nan University of Pharmacy and Science , Tainan 71710, Taiwan.
出版信息
Org Lett. 2016 Mar 18;18(6):1366-9. doi: 10.1021/acs.orglett.6b00299. Epub 2016 Mar 8.
Abstract
Citreoviridin (1) belongs to a class of F1-ATPase β-subunit inhibitors that are synthesized by highly reducing polyketide synthases. These potent mycotoxins share an α-pyrone polyene structure, and they include aurovertin, verrucosidin, and asteltoxin. The identification of the citreoviridin biosynthetic gene cluster in Aspergillus terreus var. aureus and its reconstitution using heterologous expression in Aspergillus nidulans are reported. Two intermediates were isolated that allowed the proposal of the biosynthetic pathway of citreoviridin.
摘要
黄绿青霉素（1）属于一类由高度还原型聚酮合酶合成的F1 - ATP酶β亚基抑制剂。这些强效霉菌毒素具有α - 吡喃多烯结构，包括金褐霉素、疣孢菌素和黄绿青霉素。本文报道了在土曲霉金黄色变种中黄绿青霉素生物合成基因簇的鉴定以及利用构巢曲霉中的异源表达对其进行的重构。分离出了两种中间体，据此提出了黄绿青霉素的生物合成途径。
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