Quita Salwa Mohammed
Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Electron Physician. 2016 Jan 15;8(1):1824-31. doi: 10.19082/1824. eCollection 2016 Jan.
The aim of this study was to determine the protective effects of lemon fruit extracts (LFE) against histopathological changes induced in the testes of male mice treated with cyclophosphamide (CP).
Thirty male mice were divided evenly into six groups: 1) group 1: the controls, 2) group 2: treated with LFE (10 ml/kg b wt.), 3) group 3: treated with CP (10 mg/kg b wt.), 4) group 4: treated with CP (20 mg/kg b wt.), 5) group 5: treated with LFE (10 ml/kg) + CP (10 mg/kg), 6) group 6: treated with LFE (10 ml/kg) + CP (20 mg/kg).
Histological examination of the testes of mice treated with CP revealed histopathological changes, such as atrophy, degeneration, incomplete spermatogenic series in most seminiferous tubules, and spermatogenic necrosis with pyknotic nuclei. Advanced degree of improvement was seen in testes of mice treated with LFE co-administered with CP. Most of the seminiferous tubules restored their normal structure and spermatogenic layers appeared semi-normal with complete spermatogenic series.
Lemon fruit extract in conjunction with drug treatment protects the testicular tissue against CP-induced testicular injury in mice.
本研究的目的是确定柠檬果实提取物（LFE）对环磷酰胺（CP）处理的雄性小鼠睾丸组织病理学变化的保护作用。
将30只雄性小鼠平均分为六组：1）第1组：对照组；2）第2组：用LFE处理（10毫升/千克体重）；3）第3组：用CP处理（10毫克/千克体重）；4）第4组：用CP处理（20毫克/千克体重）；5）第5组：用LFE（10毫升/千克）+ CP（10毫克/千克）处理；6）第6组：用LFE（10毫升/千克）+ CP（20毫克/千克）处理。
对用CP处理的小鼠睾丸进行组织学检查发现了组织病理学变化，如萎缩、退化、大多数生精小管中生精序列不完整以及伴有核固缩的生精坏死。在用LFE与CP联合处理的小鼠睾丸中观察到了更高程度的改善。大多数生精小管恢复了正常结构，生精层呈现半正常状态，生精序列完整。
柠檬果实提取物与药物治疗相结合可保护小鼠睾丸组织免受CP诱导的睾丸损伤。
