Torabi Fereshte, Malekzadeh Shafaroudi Majid, Rezaei Nourollah
Immunogenetics Research Center (IRC), Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Int J Reprod Biomed. 2017 Jul;15(7):403-412.
Cyclophosphamide (CP) has been known as an anticancer drug with several side effects on various organs such as a male reproductive system that can cause infertility.
To evaluate the possible combined effects of zinc oxide nanoparticles (nZno) and melatonin (Mel) on sperm parameters and histopathological changes of the testis in CP-treated rats.
42 adult male Wistar rats were divided into six groups. GI: control, GII: 60 mg/kg/wk CP, GIII and GIV, 10 mg/kg/wk Mel and 5mg/kg/wk nZno and GV: 5 mg/kg/wk nZno and 10 mg/kg/wk Mel were given 2 hr prior to CP injection, respectively,GVI: 5mg/kg/wk nZno and 10 mg/kg/wk Mel simultaneously. After 8 wk of treatment, rats were sacrificed and testis and epididymis were harvested for further evaluation.
The CP-treated group showed significant decreases in the body, testes and epididymis weights and sperm parameters (sperm count, viability, motility) with an increase abnormal sperms when compared with the control (p<0.001), as well as many histological alterations included decreased diameters of seminiferous tubules and Johnsen's Testicular Score (with degeneration, desquamation, multi-nucleated giant cell formation), whereas combined treatment (GV), showed more protective effects on CP-induced reproductive system damage compared with groups III or IV (p<0.001).
These results suggest simultaneous administration of Mel and nZno have more effectively protections against CP-induced reproductive damage than Mel or nZno alone.
环磷酰胺（CP）是一种抗癌药物，对包括男性生殖系统在内的多个器官有多种副作用，可导致不育。
评估氧化锌纳米颗粒（nZno）和褪黑素（Mel）对CP处理的大鼠精子参数和睾丸组织病理学变化的可能联合作用。
将42只成年雄性Wistar大鼠分为六组。GI：对照组，GII：60mg/kg/周CP，GIII和GIV，分别在注射CP前2小时给予10mg/kg/周Mel和5mg/kg/周nZno，GV：5mg/kg/周nZno和10mg/kg/周Mel，GVI：5mg/kg/周nZno和10mg/kg/周Mel同时给药。治疗8周后，处死大鼠，采集睾丸和附睾进行进一步评估。
与对照组相比，CP处理组的体重、睾丸和附睾重量以及精子参数（精子计数、活力、运动能力）显著降低，异常精子增加（p<0.001），同时出现许多组织学改变，包括生精小管直径减小和约翰森睾丸评分降低（伴有变性、脱屑、多核巨细胞形成），而联合治疗组（GV）与III组或IV组相比，对CP诱导的生殖系统损伤显示出更强的保护作用（p<0.001）。
这些结果表明，与单独使用Mel或nZno相比，同时给予Mel和nZno能更有效地保护CP诱导的生殖损伤。
