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从一种新描述的多瘤病毒（RacPyV）生产重组衣壳蛋白VP1，以供下游用于病毒特性分析。

Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization.

作者信息

Church Molly E, Dela Cruz Florante N, Kim Kevin, Persiani Michele, Woods Leslie W, Pesavento Patricia A, Woolard Kevin D

机构信息

UC Davis, School of Veterinary Medicine, Department of Pathology, Microbiology, and Immunology, USA.

UC Davis, School of Medicine, Department of Dermatology, USA.

出版信息

Data Brief. 2016 Feb 9;7:60-5. doi: 10.1016/j.dib.2016.01.070. eCollection 2016 Jun.

DOI:10.1016/j.dib.2016.01.070
PMID:26955649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4761659/
Abstract

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.

摘要

在此，我们描述了重组病毒衣壳蛋白的生产方法及其随后在间接酶联免疫吸附测定（ELISA）中的应用，以及用于制备兔多克隆抗体的方法。这些试剂被用于ELISA的开发和优化，该ELISA确定了野生浣熊接触一种新描述的多瘤病毒（RacPyV）的程度[1]。多克隆抗体的制备使得能够对RacPyV进行进一步的特性分析，包括免疫组织化学和免疫细胞化学技术，以回答有关该病毒发病机制的问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/1bf58ab23e2f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/e8641a4e37ff/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/c538ba76e0e2/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/1bf58ab23e2f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/e8641a4e37ff/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/c538ba76e0e2/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ff12/4761659/1bf58ab23e2f/gr3.jpg

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引用本文的文献

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Exposure to raccoon polyomavirus (RacPyV) in free-ranging North American raccoons (Procyon lotor).北美野生浣熊（北美浣熊）接触浣熊多瘤病毒（RacPyV）的情况。
Virology. 2016 Feb;489:292-9. doi: 10.1016/j.virol.2015.11.033. Epub 2016 Jan 21.

本文引用的文献

1
Exposure to raccoon polyomavirus (RacPyV) in free-ranging North American raccoons (Procyon lotor).北美野生浣熊（北美浣熊）接触浣熊多瘤病毒（RacPyV）的情况。
Virology. 2016 Feb;489:292-9. doi: 10.1016/j.virol.2015.11.033. Epub 2016 Jan 21.
2
The raccoon polyomavirus genome and tumor antigen transcription are stable and abundant in neuroglial tumors.浣熊多瘤病毒基因组和肿瘤抗原转录在神经胶质瘤中稳定且丰富。
J Virol. 2014 Nov;88(21):12816-24. doi: 10.1128/JVI.01912-14. Epub 2014 Aug 27.
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A cornucopia of human polyomaviruses.人类多瘤病毒的聚宝盆。
Nat Rev Microbiol. 2013 Apr;11(4):264-76. doi: 10.1038/nrmicro2992. Epub 2013 Mar 11.
4
Age-specific seroprevalences of merkel cell polyomavirus, human polyomaviruses 6, 7, and 9, and trichodysplasia spinulosa-associated polyomavirus.默克尔细胞多瘤病毒、人类多瘤病毒6、7和9以及棘状毛发发育异常相关多瘤病毒的年龄特异性血清阳性率。
Clin Vaccine Immunol. 2013 Mar;20(3):363-8. doi: 10.1128/CVI.00438-12. Epub 2013 Jan 9.
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The epidemiology of mouse polyoma virus infection.小鼠多瘤病毒感染的流行病学
Bacteriol Rev. 1961 Mar;25(1):18-31. doi: 10.1128/br.25.1.18-31.1961.