用于高选择性和灵敏检测生物硫化氢的双反应荧光探针
Dual-Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H2 S.
作者信息
Wei Chao, Wang Runyu, Zhang Changyu, Xu Guoce, Li Yanyan, Zhang Qiang-Zhe, Li Lu-Yuan, Yi Long, Xi Zhen
机构信息
Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin, 300071, P. R. China.
Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing, 100029, P. R. China.
出版信息
Chem Asian J. 2016 May 6;11(9):1376-81. doi: 10.1002/asia.201600262. Epub 2016 Apr 1.
Hydrogen sulfide (H2 S) is an important endogenous signaling molecule with a variety of biological functions. Development of fluorescent probes for highly selective and sensitive detection of H2 S is necessary. We show here that dual-reactable fluorescent H2 S probes could react with higher selectivity than single-reactable probes. One of the dual-reactable probes gives more than 4000-fold turn-on response when reacting with H2 S, the largest response among fluorescent H2 S probes reported thus far. In addition, the probe could be used for high-throughput enzymatic assays and for the detection of Cys-induced H2 S in cells and in zebrafish. These dual-reactable probes hold potential for highly selective and sensitive detection of H2 S in biological systems.
硫化氢（H₂S）是一种具有多种生物学功能的重要内源性信号分子。开发用于高选择性和灵敏检测H₂S的荧光探针很有必要。我们在此表明，双反应性荧光H₂S探针的反应选择性高于单反应性探针。其中一种双反应性探针与H₂S反应时产生超过4000倍的开启响应，是迄今为止报道的荧光H₂S探针中最大的响应。此外，该探针可用于高通量酶促测定以及检测细胞和斑马鱼中半胱氨酸诱导产生的H₂S。这些双反应性探针在生物系统中对H₂S进行高选择性和灵敏检测方面具有潜力。
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