一种双响应荧光探针揭示了H2O2通过胱硫醚β-合酶途径诱导的H2S生物合成。
A Dual-Response Fluorescent Probe Reveals the H2 O2 -Induced H2 S Biogenesis through a Cystathionine β-Synthase Pathway.
作者信息
Yi Long, Wei Lv, Wang Runyu, Zhang Changyu, Zhang Jie, Tan Tianwei, Xi Zhen
机构信息
State Key Laboratory of Organic-Inorganic Composites, Beijing University of Chemical Engineering (BUCT), 15 Beisanhuan East Road, Chaoyang District, Beijing 100029 (P. R. China).
Department of Chemical Biology, State Key Laboratory of Elemento-Organic Chemistry, National Engineering Research Center of Pesticide (Tianjin), Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University, Weijin Road 94, Tianjin 300071 (P. R. China).
出版信息
Chemistry. 2015 Oct 19;21(43):15167-72. doi: 10.1002/chem.201502832. Epub 2015 Sep 4.
The two signaling molecules H2 S and H2 O2 play key roles in maintaining intracellular redox homeostasis. The biological relationship between H2 O2 and H2 S remains largely unknown in redox biology. In this study, we rationally designed and synthesized single- and dual-response fluorescent probes for detecting both H2 O2 and H2 S in living cells. The dual-response probe was shown to be capable of mono- and dual-detection of H2 O2 and H2 S selectively and sensitively. Detailed bioimaging studies based on the probes revealed that both exogenous and endogenous H2 O2 could induce H2 S biogenesis in living cells. By using gene-knockdown techniques with bioimaging, the H2 S biogenesis was found to be majorly cystathionine β-synthase (CBS)-dependent. Our finding shows the first direct evidence on the biological communication between H2 O2 (ROS) and H2 S (RSS) in vivo.
两种信号分子硫化氢（H₂S）和过氧化氢（H₂O₂）在维持细胞内氧化还原稳态中起关键作用。在氧化还原生物学中，H₂O₂与H₂S之间的生物学关系在很大程度上仍不清楚。在本研究中，我们合理设计并合成了用于检测活细胞中H₂O₂和H₂S的单响应和双响应荧光探针。双响应探针能够选择性且灵敏地对H₂O₂和H₂S进行单检测和双检测。基于这些探针的详细生物成像研究表明，外源性和内源性H₂O₂均可在活细胞中诱导H₂S生物合成。通过使用基因敲除技术结合生物成像，发现H₂S生物合成主要依赖于胱硫醚β-合酶（CBS）。我们的发现首次提供了体内H₂O₂（活性氧，ROS）与H₂S（反应性硫物种，RSS）之间生物学联系的直接证据。
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