利用基于硒-硫交换反应的红色荧光探针在活细胞和斑马鱼中可视化硫化氢。
Visualizing hydrogen sulfide in living cells and zebrafish using a red-emitting fluorescent probe via selenium-sulfur exchange reaction.
机构信息
Institute of Functional Materials and Molecular Imaging, Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, College of Clinical Medicine, College of Emergency and Trauma, Hainan Medical University, Haikou, 571199, China; Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, China.
出版信息
Anal Chim Acta. 2020 May 1;1109:37-43. doi: 10.1016/j.aca.2020.02.061. Epub 2020 Feb 27.
Hydrogen sulfide (HS) is an important endogenous gasotransmitter and has been implicated with a variety of biological processes. The development of an efficient method for monitor HS fluctuations in biological systems is of great significance to understand its roles in physiological and pathological conditions. In this work, two red-emitting fluorescent probes SNARF-SSPy and SNARF-SeSPy for HS detection with turn-on fluorescence signals were reported. Interestingly, SNARF-SeSPy exhibited excellent anti-interference via dual selenium-sulfur exchange reaction even in the presence of high concentrations of thiols, whereas SNARF-SSPy did not sense HS in the same condition. Additionally, in the present of HS, SNARF-SeSPy showed a rapid response and excellent sensitivity with a detection limit of 34 nM. Most importantly, SNARF-SeSPy featured low cytotoxicity and could be employed to detect and image exogenous/endogenous HS in living cells and zebrafish.
硫化氢（HS）是一种重要的内源性气体递质，与多种生物学过程有关。开发一种有效监测生物体系中 HS 波动的方法对于了解其在生理和病理条件下的作用具有重要意义。在这项工作中，我们报道了两种用于 HS 检测的具有开启型荧光信号的红色荧光探针 SNARF-SSPy 和 SNARF-SeSPy。有趣的是，即使在高浓度巯基存在的情况下，SNARF-SeSPy 也通过双硒-硫交换反应表现出优异的抗干扰性，而 SNARF-SSPy 在相同条件下则不能检测到 HS。此外，在 HS 的存在下，SNARF-SeSPy 表现出快速响应和优异的灵敏度，检测限低至 34 nM。最重要的是，SNARF-SeSPy 具有低细胞毒性，可用于检测和成像活细胞和斑马鱼中的外源性/内源性 HS。
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