Storrer Carmen L Mueller, Deliberador Tatiana Miranda, Giovanini Allan Fernando, Crivellaro Viviane, Zielak João Cesar, Romito Giuseppe Alexandre
Graduate Program in Clinical Dentistry, Universidade Positivo, Rua: Professor Pedro Viriato Parigot de Souza, 5300, Campo Comprido, Curitiba, PR, CEP: 81280-330, Brazil.
Department of Stomatology, Division of Periodontics, School of Dentistry, Universidade de São Paulo, Av. Prof. Lineu Prestes, 2227 Cidade Universitária, São Paulo, São Paulo, Brazil.
Clin Oral Investig. 2016 Dec;20(9):2565-2573. doi: 10.1007/s00784-016-1769-4. Epub 2016 Mar 9.
The study aimed to investigate the effect of alendronate (ALN) on the inhibition of alveolar bone loss in experimental periodontitis in Wistar rats.
Periodontitis was induced by oral inoculation of Porphyromonas gingivalis with Fusobacterium nucleatum. The rats (n = 80) were randomized as follows: negative control (n = 10); positive control (n = 10); ALN groups: test 8 (n = 10), test 12 (n = 10), and test 16 (n = 10); and placebo groups: control 8 (n = 10), control 12 (n = 10), and control 16 (n = 10). Two milligrams per kilogram of ALN or placebo was administered twice weekly for 8, 12, and 16 weeks. Bone loss was determined by morphological and histological analyses. One independent, blinded examiner (ICC, 0.91) performed the measurements. The distance from the cement enamel junction to the alveolar bone crest of the second lower molar was measured: distal-vestibular (d), furca (f), mesial-vestibular (h), and area. Histometry was performed on the second contralateral molar. Sections (6 μm) were used to determine the furcation bone area (A-FB). The following statistical analyses were conducted: Mann-Whitney and Kruskal-Wallis.
PC group developed periodontitis (p < 0.0001). Morphometric analysis determined that ALN was effective in T8 for linear measurements d, f, and h (p < 0.05). No significant differences occurred at test 8, test 12, and test 16. Analysis of A-FB revealed no significant differences between the ALN and placebo groups at 8 and 16 weeks (p > 0.05). ALN was effective against bone loss in relation to A-FB after 12 weeks (p < 0.0001).
According to the methodology used, the results suggest that oral administration of ALN could influence alveolar bone loss in rats submitted to experimental periodontitis.
ALN could be a potential therapeutic approach when associated with periodontal treatment.
本研究旨在探讨阿仑膦酸钠（ALN）对抑制Wistar大鼠实验性牙周炎牙槽骨吸收的作用。
通过口腔接种牙龈卟啉单胞菌和具核梭杆菌诱导牙周炎。将80只大鼠随机分组如下：阴性对照组（n = 10）；阳性对照组（n = 10）；ALN组：试验8组（n = 10）、试验12组（n = 10）和试验16组（n = 10）；安慰剂组：对照8组（n = 10）、对照12组（n = 10）和对照16组（n = 10）。每周两次给予每千克2毫克的ALN或安慰剂，持续8、12和16周。通过形态学和组织学分析确定骨吸收情况。由一名独立的、不知情的检查者（组内相关系数，0.91）进行测量。测量从牙骨质釉质界到下颌第二磨牙牙槽嵴的距离：远中前庭侧（d）、根分叉（f）、近中前庭侧（h）和面积。对另一侧的第二磨牙进行组织计量学分析。使用6微米厚的切片确定根分叉骨面积（A-FB）。进行以下统计分析：曼-惠特尼检验和克鲁斯卡尔-沃利斯检验。
PC组发生了牙周炎（p < 0.0001）。形态计量学分析确定，ALN在试验8组对线性测量值d、f和h有效（p < 0.05）。在试验8、试验12和试验16组未发现显著差异。对A-FB的分析显示，在8周和16周时，ALN组和安慰剂组之间无显著差异（p > 0.05）。12周后，ALN对与A-FB相关的骨吸收有效（p < 0.0001）。
根据所采用的方法，结果表明口服ALN可能会影响患实验性牙周炎大鼠的牙槽骨吸收。
ALN与牙周治疗联合使用时可能是一种潜在的治疗方法。
