Hinton J C, Sidebotham J M, Gill D R, Salmond G P
Department of Biological Sciences, University of Warwick, Coventry, UK.
Mol Microbiol. 1989 Dec;3(12):1785-95. doi: 10.1111/j.1365-2958.1989.tb00164.x.
Pectate lyase (Pel) plays a crucial role in the maceration of vegetables by soft rot Erwinia spp. We have characterized the four Pel isoenzymes of Erwinia carotovora subspecies carotovora strain SCRI193. In this paper we concentrate on two isoenzymes which have different locations in SCRI193: PLb is periplasmic and PLc is extracellular. Comparison of the gene products and nucleotide sequences of pelB and pelC allowed us to assign them to different gene families. In addition, we have identified a number of conserved amino acid residues that are common to all extracellular Pel isoenzymes.
果胶酸裂解酶（Pel）在软腐欧文氏菌属细菌对蔬菜的浸解过程中起着关键作用。我们已经对胡萝卜软腐欧文氏菌胡萝卜亚种菌株SCRI193的四种Pel同工酶进行了特性分析。在本文中，我们着重研究在SCRI193中位于不同位置的两种同工酶：PLb位于周质空间，PLc位于细胞外。对pelB和pelC的基因产物及核苷酸序列进行比较，使我们能够将它们归属于不同的基因家族。此外，我们还鉴定出了所有细胞外Pel同工酶共有的一些保守氨基酸残基。
