Zhang W H, Bai X F, Nie D K, Deng Q D
Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.
Microbiol Immunol. 1989;33(12):1039-43.
For the purpose of improving the procedures of identification of leptospires, a set of 5 monoclonal antibodies with different serological reactivity against serovars of Leptospira interrogans Icterohaemorrhagiae serogroup isolated in China was developed. One hundred and eight strains isolated from epidemic fields in 5 provinces in southern China were distinctly identified into 4 serovars of Icterohaemorrhagiae serogroup by the monoclonal antibody procedure, i.e., 98 isolates were identified as serovar lai, 7 as icterohaemorrhagiae, 2 as copenhageni, and 1 as H2. Factor antiserum procedure was used at the same time as control for typing these strains and an identical result was obtained.
为改进钩端螺旋体的鉴定程序，研制了一组5种单克隆抗体，它们对在中国分离的问号钩端螺旋体黄疸出血血清群的血清型具有不同的血清学反应性。用单克隆抗体程序将从中国南方5省流行区分离出的108株菌株明确鉴定为黄疸出血血清群的4个血清型，即98株鉴定为赖型，7株为黄疸出血型，2株为哥本哈根型，1株为H2型。同时采用因子抗血清程序作为这些菌株分型的对照，获得了相同的结果。
