Harashima S, Shimada Y, Nakade S, Oshima Y
Department of Fermentation Technology, Osaka University, Japan.
Mol Gen Genet. 1989 Nov;219(3):495-8. doi: 10.1007/BF00259627.
A mutant plasmid, pX, derived from the 1453 base pair small plasmid, YARp1 (or TRP1 RI circle), consists of 849 base pairs of DNA bearing the TRP1 gene and the ARS1 sequence of Saccharomyces cerevisiae and, unlike YARp1 and other commonly used yeast plasmids, highly multimerizes in a S. cerevisiae host. The multimerization of pX was dependent on RAD52, which is known to be necessary for homologous recombination in S. cerevisiae. Based upon this observation, a regulated system of multimerization of pX with GAL1 promoter-driven RAD52 has been developed. We conclude that the regulated multimerization of pX could provide a useful model system to study genetic recombination in the eukaryotic cell, in particular to investigate recombination intermediates and the effects of various trans-acting mutations on the multimerization and recombination of plasmids.
一种突变质粒pX，源自1453个碱基对的小质粒YARp1（或TRP1 RI环），由849个碱基对的DNA组成，带有酿酒酵母的TRP1基因和ARS1序列，并且与YARp1及其他常用酵母质粒不同，它在酿酒酵母宿主中高度多聚化。pX的多聚化依赖于RAD52，已知RAD52是酿酒酵母中同源重组所必需的。基于这一观察结果，已开发出一种由GAL1启动子驱动RAD52的pX多聚化调控系统。我们得出结论，pX的调控多聚化可为研究真核细胞中的基因重组提供一个有用的模型系统，特别是用于研究重组中间体以及各种反式作用突变对质粒多聚化和重组的影响。
