Cohen A, Laban A
Mol Gen Genet. 1983;189(3):471-4. doi: 10.1007/BF00325911.
Interplasmidic and intraplasmidic recombination proficiencies were determined in E. coli bacterial strains carrying rec mutations. Our results defined the role of recF gene function, recB, recC, and sbcB gene products (exonuclease V and exonuclease I) in plasmidic recombination in wild-type E. coli cells and in cells in which the recE recombination pathway is activated. RecF gene function is required for interplasmidic recombination regardless of the recB recC genotype. Intraplasmidic recombination is recF dependent in cells having a functional exonuclease V, but not in recB recC mutants. Exonuclease V activity inhibits both interplasmidic and intraplasmidic recombination via the recE pathway.
在携带rec突变的大肠杆菌菌株中测定了质粒间和质粒内的重组能力。我们的结果确定了recF基因功能、recB、recC和sbcB基因产物（外切核酸酶V和外切核酸酶I）在野生型大肠杆菌细胞以及recE重组途径被激活的细胞中的质粒重组中的作用。无论recB recC基因型如何，质粒间重组都需要RecF基因功能。在具有功能性外切核酸酶V的细胞中，质粒内重组依赖于recF，但在recB recC突变体中则不然。外切核酸酶V活性通过recE途径抑制质粒间和质粒内的重组。
