Song D D, Rossier J, Harlan R E
Department of Anatomy, Tulane University School of Medicine, New Orleans, LA 70112.
Peptides. 1989 Nov-Dec;10(6):1239-46. doi: 10.1016/0196-9781(89)90018-1.
Immunocytochemistry using an antiserum to the C-terminal octapeptide of synenkephalin, proenkephalin(63-70), was performed throughout the rat brain and revealed numerous immunopositive fibers and some cell bodies. The morphology and distribution of synenkephalin immunoreactivity was extremely similar to that of a commercial methionine enkephalin (Met-ENK) antiserum. Colchicine pretreatment allowed the immunostaining of cell bodies not otherwise possible without pretreatment, but did not affect the distribution of immunoreactive fibers. Using 6 microns serial sections, we were able to colocalize synenkephalin and Met-ENK immunoreactivities in gigantocellular neurons of the medullary reticular formation. Preabsorption of the antiserum with [Tyr63]proenkephalin(63-70) octapeptide (YEESHLLA) completely eliminated immunoreactivity in the rat brain, while preabsorption with all other peptides used had no detectable effect. We conclude that our antiserum to synenkephalin is specific for enkephalinergic cell bodies, fibers and terminals. The synenkephalin antiserum used in these studies may have advantages over other antisera utilized for immunocytochemical detection of proenkephalin gene expression.
使用抗脑啡肽原（63 - 70）C末端八肽的抗血清进行免疫细胞化学实验，该实验在整个大鼠脑内进行，结果显示有大量免疫阳性纤维和一些细胞体。脑啡肽免疫反应性的形态和分布与市售的甲硫氨酸脑啡肽（Met - ENK）抗血清极为相似。秋水仙碱预处理使得在未预处理时无法实现的细胞体免疫染色成为可能，但并未影响免疫反应性纤维的分布。使用6微米连续切片，我们能够在延髓网状结构的巨细胞神经元中使脑啡肽和Met - ENK免疫反应性共定位。用[酪氨酸63]脑啡肽原（63 - 70）八肽（YEESHLLA）对该抗血清进行预吸附，完全消除了大鼠脑内的免疫反应性，而用其他所有使用的肽进行预吸附均未产生可检测到的影响。我们得出结论，我们的脑啡肽抗血清对脑啡肽能细胞体、纤维和终末具有特异性。这些研究中使用的脑啡肽抗血清可能比用于免疫细胞化学检测脑啡肽原基因表达的其他抗血清具有优势。
