Darwish Hany W, Bakheit Ahmed H, Abdelhameed Ali S
Acta Pharm. 2016 Mar;66(1):83-95. doi: 10.1515/acph-2016-0004.
Simultaneous spectrophotometric analysis of a multi-component dosage form of olmesartan, amlodipine and hydrochlorothiazide used for the treatment of hypertension has been carried out using various chemometric methods. Multivariate calibration methods include classical least squares (CLS) executed by net analyte processing (NAP-CLS), orthogonal signal correction (OSC-CLS) and direct orthogonal signal correction (DOSC-CLS) in addition to multivariate curve resolution-alternating least squares (MCR-ALS). Results demonstrated the efficiency of the proposed methods as quantitative tools of analysis as well as their qualitative capability. The three analytes were determined precisely using the aforementioned methods in an external data set and in a dosage form after optimization of experimental conditions. Finally, the efficiency of the models was validated via comparison with the partial least squares (PLS) method in terms of accuracy and precision.
采用多种化学计量学方法对用于治疗高血压的奥美沙坦、氨氯地平和氢氯噻嗪多组分剂型进行了同时分光光度分析。多元校准方法包括通过净分析物处理（NAP-CLS）执行的经典最小二乘法（CLS）、正交信号校正（OSC-CLS）和直接正交信号校正（DOSC-CLS），此外还有多元曲线分辨交替最小二乘法（MCR-ALS）。结果证明了所提出方法作为定量分析工具的有效性及其定性能力。在优化实验条件后，使用上述方法在外部数据集和剂型中精确测定了三种分析物。最后，通过与偏最小二乘法（PLS）在准确性和精密度方面进行比较，验证了模型的有效性。
