Kinoshita Takamasa, Kiso Keita, LE Dung Q, Shiigi Hiroshi, Nagaoka Tsutomu
Department of Applied Chemistry, Osaka Prefecture University.
Anal Sci. 2016;32(3):301-5. doi: 10.2116/analsci.32.301.
Metal nanoparticles express unique light-scattering characteristics based on the localized surface plasmon resonance, which depends on the metal species, particle size, and aggregation state of the nanoparticles. Therefore, we focused on the light-scattering characteristics of metal nanoparticles, such as silver, gold, and copper oxide, adsorbed on a bacterium. Monodisperse silver nanoparticles expressed the strongest scattered light among them, and showed various colors of scattered light. Although a monodisperse gold nanoparticle produced monochromatic light (green color), the color of the scattered light strongly depended on the aggregation state of the nanoparticles on a bacterium. On the other hand, copper oxide nanoparticles expressed monochromatic light (blue color), regardless of their aggregation states on a bacterium. We examined details concerning the light-scattering characteristics of metal nanoparticles, and discussed the possibility of their applications to bacterial cell imaging.
金属纳米颗粒基于局域表面等离子体共振表现出独特的光散射特性，这取决于金属种类、颗粒大小以及纳米颗粒的聚集状态。因此，我们重点研究了吸附在细菌上的金属纳米颗粒（如银、金和氧化铜）的光散射特性。单分散银纳米颗粒在其中表现出最强的散射光，并呈现出各种颜色的散射光。尽管单分散金纳米颗粒产生单色光（绿色），但散射光的颜色强烈依赖于细菌上纳米颗粒的聚集状态。另一方面，氧化铜纳米颗粒无论在细菌上的聚集状态如何，都表现出单色光（蓝色）。我们研究了金属纳米颗粒光散射特性的细节，并讨论了它们应用于细菌细胞成像的可能性。
