Ito Yoichi, Itoh Naoyuki, Kimura Yuya, Kanai Kazutaka
Ito Animal Hospital, Gakuendai 2-2-1, Miyashiro, Minami Saitama, Saitama, 345-0826, Japan.
Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Kitasato University, Higashi 23-35-1, Towada, Aomori, 034-8628, Japan.
Parasitol Res. 2016 May;115(5):2121-3. doi: 10.1007/s00436-016-4984-7. Epub 2016 Mar 10.
Cryptosporidium spp. are pathogenic protozoan that can cause gastrointestinal illness in mammalian hosts. As a result of the close contact between humans and cats, there is concern regarding the potential zoonotic transmission of Cryptosporidium spp. from infected cats; however, few data have been reported regarding the prevalence of this pathogen among cats. Here, we report the prevalence of Cryptosporidium spp. among breeding cattery cats in Japan. A total of 286 fresh fecal samples were collected from breeding cattery cats at seven facilities located across Japan. A nested polymerase chain reaction (PCR) assay targeting the 18S rRNA gene was employed for the detection of Cryptosporidium spp. Four cats (1.4 %), from two catteries, were positive for Cryptosporidium spp. Age and fecal condition were not significantly associated with prevalence. The four positive samples displayed 99-100 % sequence similarity to Cryptosporidium felis sequences. Our findings indicated that the prevalence of Cryptosporidium spp. was low among breeding cattery cats in Japan, and therefore the risk of zoonotic transmission to humans was also likely to be low.
隐孢子虫属是可在哺乳动物宿主中引起胃肠道疾病的致病性原生动物。由于人类与猫的密切接触，人们担心隐孢子虫属会从受感染的猫身上发生潜在的人畜共患传播；然而，关于这种病原体在猫中的流行情况，鲜有数据报道。在此，我们报告了日本繁殖猫舍中猫隐孢子虫属的流行情况。从日本各地七个设施的繁殖猫舍猫中总共采集了286份新鲜粪便样本。采用针对18S rRNA基因的巢式聚合酶链反应（PCR）检测法来检测隐孢子虫属。来自两个猫舍的四只猫（1.4%）隐孢子虫属检测呈阳性。年龄和粪便状况与流行率无显著关联。这四个阳性样本与猫隐孢子虫序列的序列相似性为99 - 100%。我们的研究结果表明，日本繁殖猫舍中猫隐孢子虫属的流行率较低，因此人畜共患传播给人类的风险也可能较低。
