通过计算最大荧光下降值进行犬淋巴瘤的熔解曲线分析。
Melting curve analysis in canine lymphoma by calculating maximum fluorescence decrease.
机构信息
Clinic of Small Animal Medicine, Center for Clinical Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany.
Department of Pathobiology, Clinical Pathology, University of Veterinary Medicine, Vienna, Austria.
出版信息
Vet Comp Oncol. 2017 Jun;15(2):563-575. doi: 10.1111/vco.12200. Epub 2016 Mar 9.
PARR is widely used in the diagnostics of canine lymphoma. In human and veterinary medicine, melting curve analysis (MCA) has successfully been introduced to facilitate the process. Since visual interpretation of melting curves can be rather subjective, the purpose of this study was to develop an objective interpretation of melting curves by calculating the maximum fluorescence decrease (dF ) within a defined rise of temperature. Lymph node aspirates and blood of 34 dogs with lymphoma and 28 control dogs were tested. 27/34 lymphoma cases were correctly detected to be monoclonal (sensitivity 79%). 2/28 control dogs showed a monoclonal rearrangement (specificity 93%). B- and T-cell neoplasia were still detectable using DNA amount as low as 10 ng. In serial dilutions of tumor DNA with DNA of normal tonsils, the detection limit was 25% for B-cell lymphomas and 100% for T-cell lymphoma, suggesting that PCR conditions could still be optimized.
PARR 广泛应用于犬淋巴瘤的诊断。在人类和兽医医学中，熔解曲线分析（MCA）已成功引入，以简化这一过程。由于熔解曲线的视觉解释可能具有主观性，本研究旨在通过计算特定温度上升范围内的最大荧光下降（dF）来对熔解曲线进行客观解释。对 34 只患有淋巴瘤的犬和 28 只对照犬的淋巴结抽吸物和血液进行了检测。27/34 例淋巴瘤病例被正确检测为单克隆（敏感性 79%）。2/28 只对照犬显示单克隆重排（特异性 93%）。使用低至 10 ng 的 DNA 量，仍可检测 B 细胞和 T 细胞肿瘤。在肿瘤 DNA 与正常扁桃体 DNA 的连续稀释中，B 细胞淋巴瘤的检测限为 25%，T 细胞淋巴瘤的检测限为 100%，这表明 PCR 条件仍可优化。
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