Ohmura S, Leipig M, Schöpper I, Hergt F, Weber K, Rütgen B C, Tsujimoto H, Hermanns W, Hirschberger J
Clinic of Small Animal Medicine, Center for Clinical Veterinary Medicine, Ludwig Maximilian University, Munich, Germany.
Institute of Veterinary Pathology, Ludwig Maximilian University, Munich, Germany.
Vet Comp Oncol. 2017 Mar;15(1):194-207. doi: 10.1111/vco.12151. Epub 2015 May 18.
The diagnosis of canine intestinal lymphoma by morphological examination is challenging, especially when endoscopic tissue specimens are used. The utility of detection of antigen receptor gene rearrangement by polymerase chain reaction (PARR) in canine lymphoma has been well established, but its usefulness to distinguish enteritis and intestinal lymphoma remains unclear. In this retrospective study we assessed clonality of 29 primary canine intestinal lymphoma, 14 enteritis and 15 healthy control cases by PARR analysis, using formalin-fixed, paraffin-embedded full-thickness tissue specimens. We could detect monoclonal rearrangements in 22 of 29 canine intestinal lymphomas [76%; 95% confidence interval (CI) 56-90%] and polyclonal rearrangements in all of the enteritis and healthy control cases (100%; CI 88-100%). We revealed a predominance of T-cell phenotype compared to B-cell phenotype (85%; CI 65-96% and 15%; CI 4-35%, respectively). We showed that PARR analysis contributes to differentiation of canine intestinal lymphoma from enteritis and to phenotyping of lymphomas.
通过形态学检查诊断犬肠道淋巴瘤具有挑战性，尤其是在内镜组织标本用于诊断时。聚合酶链反应（PARR）检测抗原受体基因重排在犬淋巴瘤中的应用已得到充分证实，但其在区分肠炎和肠道淋巴瘤方面的作用仍不明确。在这项回顾性研究中，我们使用福尔马林固定、石蜡包埋的全层组织标本，通过PARR分析评估了29例原发性犬肠道淋巴瘤、14例肠炎和15例健康对照病例的克隆性。我们在29例犬肠道淋巴瘤中的22例中检测到单克隆重排[76%；95%置信区间（CI）56 - 90%]，在所有肠炎和健康对照病例中检测到多克隆重排（100%；CI 88 - 100%）。与B细胞表型相比，我们发现T细胞表型占优势（分别为85%；CI 65 - 96%和15%；CI 4 - 35%）。我们表明，PARR分析有助于区分犬肠道淋巴瘤与肠炎，并有助于淋巴瘤的表型分析。
