Lee Dong Hoon, Moon Jihye, Ryu Jinhyun, Jeong Joo Yeon, Roh Gu Seob, Kim Hyun Joon, Cho Gyeong Jae, Choi Wan Sung, Kang Sang Soo
Department of Anatomy & Convergence Medical Science, Institute of Health Sciences, School of Medicine,Gyeongsang National University.
Genes Genet Syst. 2016 Apr 28;90(6):335-42. doi: 10.1266/ggs.15-00026. Epub 2016 Mar 10.
Fetal alcohol syndrome (FAS) is a condition resulting from excessive drinking by pregnant women. Symptoms of FAS include abnormal facial features, stunted growth, intellectual deficits and attentional dysfunction. Many studies have investigated FAS, but its underlying mechanisms remain unknown. This study evaluated the relationship between alcohol exposure during the synaptogenesis period in postnatal mice and subsequent cognitive function in adult mice. We delivered two injections, separated by 2 h, of ethanol (3 g/kg, ethanol/saline, 20% v/v) to ICR mice on postnatal day 7. After 10 weeks, we conducted a behavioral test, sacrificed the animals, harvested brain tissue and analyzed hippocampal gene expression using a microarray. In ethanol-treated mice, there was a reduction in brain size and decreased neuronal cell number in the cortex, and also cognitive impairment. cDNA microarray results indicated that 1,548 genes showed a > 2-fold decrease in expression relative to control, whereas 974 genes showed a > 2-fold increase in expression relative to control. Many of these genes were related to signal transduction, synaptogenesis and cell membrane formation, which are highlighted in our findings.
胎儿酒精综合征（FAS）是一种由孕妇过度饮酒导致的病症。FAS的症状包括面部特征异常、生长发育迟缓、智力缺陷和注意力功能障碍。许多研究对FAS进行了调查，但其潜在机制仍不清楚。本研究评估了出生后小鼠突触发生期酒精暴露与成年小鼠后续认知功能之间的关系。我们在出生后第7天给ICR小鼠注射两次乙醇（3 g/kg，乙醇/生理盐水，20% v/v），两次注射间隔2小时。10周后，我们进行了行为测试，处死动物，采集脑组织，并使用微阵列分析海马体基因表达。在乙醇处理的小鼠中，脑体积减小，皮层神经元细胞数量减少，同时存在认知障碍。cDNA微阵列结果表明，相对于对照组，1548个基因的表达下降了2倍以上，而974个基因的表达相对于对照组增加了2倍以上。我们的研究结果突出显示，这些基因中有许多与信号转导、突触发生和细胞膜形成有关。
