施氏榄仁（Hoschst. Ex Rich）ENG. 叶片甲醇提取物的植物化学、抗伤害感受及抗炎活性

Phytochemistry, Antinociceptive and Anti-inflammatory Actvities of Methanolic Leaves Extract of Lannea schimperi (Hoschst. Ex Rich) ENG.

作者信息

Egbe Emmanuel O, Akumka David D, Adamu Mohammed, Mikail Hudu G

机构信息

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Abuja, Abuja, Nigeria.

出版信息

Recent Pat Biotechnol. 2016;9(2):145-52. doi: 10.2174/187220830902160308195034.

DOI:10.2174/187220830902160308195034

PMID:26961671

Abstract

BACKGROUND

Pain whether acute or chronic, peripheral or central is believed to originate from inflammation and inflammatory response. The potential toxicity of antinociceptive and antiinflammatory agents such as non steroidal anti-inflammatory drugs (NSAIDs) necessitates sourcing for less toxic alternative drugs. Many natural substances have been used for the management of pain and inflammation with success traditionally.

METHODS

The present study sought to investigate phytochemistry, antinociceptive and anti-inflammatory potentials of the methanolic leaves extract of Lannea schimperi. Preliminary phytochemical test was conducted on the extract. Antinociceptive and anti-inflammatory potentials of the extract at doses of 12 and 24 mg/kg were evaluated using acetic acid induced writhing model in mice and egg albumin induced acute inflammation model in rats' respectively. Aspirin at dose of 80 mg/kg was used as the standard drug given to the positive control group, while the drug vehicle was given to the negative control group.

RESULT

Phytochemical analysis revealed the presence of carbohydrate, glycoside, reducing sugars, triterpene, steroids, cardiac glycosides, flavonoids, phenolic glycosides, alkaloids, tannins, condensed tannins and saponins. The data obtained showed that at both doses the extract significantly (P< 0.05) decreased the acetic acid induced writhing reflex in mice when compared to the negative control group. The result also demonstrated significant (P< 0.05) anti-inflammatory activity of the extract at both doses. However, there was no significant difference (P> 0.05) between the extract treated groups and the group treated with the standard drug (positive control).

CONCLUSION

The patent data revealed the antinociceptive and anti-inflammatory potentials of the methanolic leaves extract of Lannea schimperi which could be beneficial in alleviating painful inflammatory conditions.

摘要

背景

疼痛，无论是急性还是慢性，外周性还是中枢性，都被认为源于炎症和炎症反应。抗伤害感受和抗炎药物（如非甾体抗炎药）的潜在毒性使得有必要寻找毒性较小的替代药物。许多天然物质传统上已成功用于疼痛和炎症的管理。

方法

本研究旨在调查辛氏榄仁叶甲醇提取物的植物化学、抗伤害感受和抗炎潜力。对提取物进行了初步植物化学测试。分别使用小鼠醋酸诱导扭体模型和大鼠蛋清诱导急性炎症模型，评估了剂量为12和24mg/kg时提取物的抗伤害感受和抗炎潜力。80mg/kg剂量的阿司匹林用作阳性对照组的标准药物，而阴性对照组给予药物载体。

结果

植物化学分析显示存在碳水化合物、糖苷、还原糖、三萜、甾体、强心苷、黄酮类、酚苷、生物碱、单宁、缩合单宁和皂苷。获得的数据表明，与阴性对照组相比，在两个剂量下提取物均显著（P<0.05）降低了小鼠醋酸诱导的扭体反射。结果还表明，在两个剂量下提取物均具有显著（P<0.05）的抗炎活性。然而，提取物处理组与标准药物处理组（阳性对照组）之间没有显著差异（P>0.05）。