Bhuiya N M Mahmudul Alam, Hasan Mahmud, Mahmud Zobaer Al, Qais Nazmul, Kabir Mohammad Shah Hafez, Ahmed Farhan, Uddin Mir Muhammad Nasir
J Complement Integr Med. 2017 Mar 1;14(1). doi: 10.1515/jcim-2016-0040.
Background The objective of the study was to evaluate the antinociceptive, antipyretic and anti-inflammatory activities of ethanolic extract, methanolic extract and n-hexane and chloroform-soluble fractions of methanolic extract of Eria javanica leaves in animal model (rat and mice). Methods The anti-nociceptive potentials of the extracts were studied using the acetic acid-induced writhing test in mice and the antipyretic activity was investigated using yeast-induced pyrexia in rats. Anti-inflammatory activity test was done on rats at a dose by using carrageenan-induced paw edema test. Results In acetic acid-induced writhing inhibition study in Swiss albino mice, the crude methanolic extract at 200 mg/kg and 400 mg/kg doses and the n-hexane soluble fraction of crude methanolic extract at 400 mg/kg showed statistically significant activity with 53.21 % (p<0.001), 50.36 % (p<0.001) and 67.86 % (p<0.001) inhibition respectively compared to control. The crude ethanolic extract showed statistically significant antipyretic activity from 1 hours and onwards after administration at doses of 200 mg/kg body weight (p<0.005 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively) and 400 mg/kg body weight (p<0.05 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively). The crude methanolic extract showed statistically significant antipyretic activity from 2 hours and onwards at 400 mg/kg body weight (p<0.05 at 2nd hour and p<0.001 at 3rd and 4th hour respectively) and 200 mg/kg body weight dose showed statistically significant antipyretic activity from 3 hours and onward(p<0.001) in Brewer's yeast-induced pyrexia test in albino Wister rats. In carrageenan-induced rat's paw edema test, crude methanolic extract showed statistically significant anti-inflammatory activity from 2nd hour and onwards. The chloroform-soluble fraction of methanolic extract also showed significant activity from 1st hour onwards. Conclusions This study thereby indicates that leaves of E. javanica possess peripheral analgesic, antipyretic and anti-inflammatory activities and therefore a suitable candidate for further study.
背景 本研究的目的是在动物模型（大鼠和小鼠）中评估爪哇叶乙醇提取物、甲醇提取物以及甲醇提取物的正己烷和氯仿可溶部分的抗伤害感受、解热和抗炎活性。方法 使用小鼠醋酸诱导扭体试验研究提取物的抗伤害感受潜力，使用酵母诱导大鼠发热来研究解热活性。通过角叉菜胶诱导大鼠足爪肿胀试验，以一定剂量对大鼠进行抗炎活性测试。结果 在瑞士白化小鼠醋酸诱导扭体抑制研究中，200mg/kg和400mg/kg剂量的粗甲醇提取物以及400mg/kg的粗甲醇提取物正己烷可溶部分显示出统计学显著活性，与对照组相比，抑制率分别为53.21%（p<0.001）、50.36%（p<0.001）和67.86%（p<0.001）。在白化Wistar大鼠酵母诱导发热试验中，200mg/kg体重剂量的粗乙醇提取物在给药后1小时及以后显示出统计学显著的解热活性（第1小时p<0.005，第2、3和4小时p<0.001），400mg/kg体重剂量也如此（第1小时p<0.05，第2、3和4小时p<0.001）。400mg/kg体重剂量的粗甲醇提取物在2小时及以后显示出统计学显著的解热活性（第2小时p<0.05，第3和4小时p<0.001），200mg/kg体重剂量在3小时及以后显示出统计学显著的解热活性（p<0.001）。在角叉菜胶诱导的大鼠足爪肿胀试验中，粗甲醇提取物在第2小时及以后显示出统计学显著的抗炎活性。甲醇提取物的氯仿可溶部分从第1小时起也显示出显著活性。结论 本研究因此表明爪哇叶具有外周镇痛、解热和抗炎活性，因此是进一步研究的合适候选物。
