Hale I, Zhang X, Fu D, Dubcovsky J
Department of Plant Sciences, University of California - Davis, Davis, CA 95616.
Department of Agronomy, Shandong Agricultural University, Tai'an, Shandong, China, 271018.
J Plant Regist. 2012 Oct 4;7(1):108-112. doi: 10.3198/jpr2012.03.0150crg.
While the high-temperature adult plant resistance gene represents a promising source of quantitative and potentially race non-specific resistance to wheat stripe rust (causal organism Westend. f. sp. ), its tight linkage (0.3 cM) with the high-grain protein content gene may hinder its introgression in certain cases, such as in soft wheat varieties requiring low grain protein content or in lines where the allele may be associated with a yield penalty. The development and registration of two donor lines, one tetraploid ( L. ssp. ; PI 656793) and one hexaploid ( L. ssp. ; PI 664549), each carrying the resistant wild emmer ( ssp. ) allele for linked with the non-functional allele, are intended to overcome this potential limitation. Meiotic recombination events breaking the linkage between these two genes were discovered during the systematic screening of a population of 4,500 F durum plants (cv. Langdon background) used to fine map . One of the critical recombination events was selected for fixation by self-pollination and transferred to a California adapted spring hexaploid background (breeding line UC1110) through five generations of backcrossing. Genotypic and phenotypic data confirm the presence of and the non-functional allele in both registered lines.
虽然高温成株抗性基因是小麦条锈病(病原菌为Westend. f. sp.)数量抗性和潜在小种非特异性抗性的一个有前景的来源,但其与高 grain 蛋白含量基因紧密连锁(0.3 cM),在某些情况下可能会阻碍其导入,例如在需要低 grain 蛋白含量的软质小麦品种中,或者在 等位基因可能与产量损失相关的品系中。两个供体系的开发和登记,一个是四倍体(L. ssp.;PI 656793),另一个是六倍体(L. ssp.;PI 664549),每个都携带与无功能 等位基因连锁的抗野生二粒小麦(ssp.) 等位基因,旨在克服这一潜在限制。在对用于精细定位 的4500个F硬粒小麦植株(cv. Langdon背景)群体进行系统筛选期间,发现了打破这两个基因之间连锁的减数分裂重组事件。通过自花授粉选择了其中一个关键重组事件进行固定,并通过五代回交转移到加利福尼亚适应的春性六倍体背景(育种系UC1110)中。基因型和表型数据证实了两个登记系中 等位基因和无功能 等位基因的存在。