Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164-6420, USA,
Theor Appl Genet. 2015 Jun;128(6):1083-101. doi: 10.1007/s00122-015-2492-2. Epub 2015 Mar 10.
Potential novel and known QTL for race-specific all-stage and adult plant resistance to stripe rust were identified by genome-wide association mapping in the US PNW winter wheat accessions. Stripe rust (Puccinia striiformis F. sp. tritici; also known as yellow rust) is a globally devastating disease of wheat (Triticum aestivum L.) and a major threat to wheat production in the US Pacific Northwest (PNW), therefore both adult plant and all-stage resistance have been introduced into the winter wheat breeding programs in the PNW. The goal of this study was to identify quantitative trait loci (QTL) and molecular markers for these resistances through genome-wide association (GWAS) mapping in winter wheat accessions adapted to the PNW. Stripe rust response for adult plants was evaluated in naturally occurring epidemics in a total of nine environments in Washington State, USA. Seedling response was evaluated with three races under artificial inoculation in the greenhouse. The panel was genotyped with the 9K Illumina Wheat single nucleotide polymorphism (SNP) array and additional markers linked to previously reported genes and QTL for stripe rust resistance. The population was grouped into three sub-populations. Markers linked to Yr17 and previously reported QTL for stripe rust resistance were identified on chromosomes 1B, 2A, and 2B. Potentially novel QTL associated with race-specific seedling response were identified on chromosomes 1B and 1D. Potentially novel QTL associated with adult plant response were located on chromosomes 2A, 2B, 3B, 4A, and 4B. Stripe rust was reduced when multiple alleles for resistance were present. The resistant allele frequencies were different among sub-populations in the panel. This information provides breeders with germplasm and closely linked markers for stripe rust resistance to facilitate the transfer of multiple loci for durable stripe rust resistance into wheat breeding lines and cultivars.
在美国太平洋西北地区的冬小麦品种中，通过全基因组关联图谱分析，鉴定出与特定条锈病全生育期和成株期抗性相关的新的和已知的 QTL。条锈病（Puccinia striiformis F. sp. tritici；也称为黄锈病）是一种全球性的小麦毁灭性病害，也是美国太平洋西北地区（PNW）小麦生产的主要威胁，因此，成株期和全生育期抗性都已被引入 PNW 的冬小麦育种计划中。本研究的目的是通过对适应 PNW 的冬小麦品种进行全基因组关联（GWAS）图谱分析，鉴定这些抗性的数量性状基因座（QTL）和分子标记。在华盛顿州的九个环境中，通过自然发生的流行病情况评估了成株期的条锈病反应。在温室中，通过三个菌系的人工接种评估了幼苗的反应。该群体使用 Illumina 小麦 9K 单核苷酸多态性（SNP）阵列和与条锈病抗性相关的先前报道的基因和 QTL 的其他标记进行了基因型分析。该群体分为三个亚群。在染色体 1B、2A 和 2B 上鉴定到与 Yr17 相关的标记和先前报道的条锈病抗性 QTL。在染色体 1B 和 1D 上鉴定到与幼苗反应特异性相关的潜在新 QTL。与成株期反应相关的潜在新 QTL 位于染色体 2A、2B、3B、4A 和 4B 上。当存在多个抗性等位基因时，条锈病的严重程度降低。该群体中不同亚群的抗性等位基因频率不同。这些信息为育种者提供了具有条锈病抗性的种质资源和紧密连锁的标记，以促进将多个抗条锈病基因位点转移到小麦育种系和品种中，从而获得持久的抗条锈病能力。
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