养鱼场沉积物中存在鱼类病原体鲑鱼弧菌。
Presence of the fish pathogen Vibrio salmonicida in fish farm sediments.
作者信息
Enger O, Husevåg B, Goksøyr J
机构信息
Department of Microbiology and Plant Physiology, University of Bergen, Norway.
出版信息
Appl Environ Microbiol. 1989 Nov;55(11):2815-8. doi: 10.1128/aem.55.11.2815-2818.1989.
Abstract
The persistence of the fish pathogen Vibrio salmonicida in fish farm sediments was studied by use of fluorescent-antibody techniques. The specificities of the monoclonal antibodies and polyclonal rabbit serum used in the study were tested against a number of Vibrio strains, including 4 isolates from intestinal tracts of healthy fish and 98 isolates from sediments. V. salmonicida was detected in sediment samples from diseased farms several months after an outbreak of the disease. The bacterium was also detected in a sediment sample from a disease-free fish farm. No V. salmonicida could be detected in sediments not influenced by fish farming. The number of positive samples was generally higher with application of rabbit serum as opposed to use of monoclonal antibodies, indicating that the rabbit serum may cross-react with other bacteria.
摘要
利用荧光抗体技术研究了鱼类病原体杀鲑气单胞菌在养鱼场沉积物中的持久性。该研究中使用的单克隆抗体和兔多克隆血清的特异性针对多种弧菌菌株进行了测试，包括从健康鱼肠道分离出的4株菌株和从沉积物中分离出的98株菌株。在疾病爆发数月后，在患病养殖场的沉积物样本中检测到了杀鲑气单胞菌。在一个无病养鱼场的沉积物样本中也检测到了这种细菌。在未受养鱼业影响的沉积物中未检测到杀鲑气单胞菌。与使用单克隆抗体相比，使用兔血清时阳性样本数量通常更高，这表明兔血清可能与其他细菌发生交叉反应。
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