Kurokawa Masaki, King Patrick M, Wu Xiaoge, Joyce Eadaoin M, Mason Timothy J, Yamamoto Ken
Department of Pure and Applied Physics, Faculty of Engineering Science, Kansai University, Osaka 564-8680, Japan.
The Sonochemistry Group, Faculty of Health and Life Sciences, Coventry University, Coventry CV1 5FB, United Kingdom.
Ultrason Sonochem. 2016 Jul;31:157-62. doi: 10.1016/j.ultsonch.2015.12.011. Epub 2015 Dec 19.
In this study, the efficiency of ultrasonic disruption of Chaetoceros gracilis, Chaetoceros calcitrans, and Nannochloropsis sp. was investigated by applying ultrasonic waves of 0.02, 0.4, 1.0, 2.2, 3.3, and 4.3 MHz to algal suspensions. The results showed that reduction in the number of algae was frequency dependent and that the highest efficiency was achieved at 2.2, 3.3, and 4.3MHz for C. gracilis, C. calcitrans, and Nannochloropsis sp., respectively. A review of the literature suggested that cavitation, rather than direct effects of ultrasonication, are required for ultrasonic algae disruption, and that chemical effects are likely not the main mechanism for algal cell disruption. The mechanical resonance frequencies estimated by a shell model, taking into account elastic properties, demonstrated that suitable disruption frequencies for each alga were associated with the cell's mechanical properties. Taken together, we consider here that physical effects of ultrasonication were responsible for algae disruption.
在本研究中，通过对纤细角毛藻、钙板角毛藻和微拟球藻的藻悬液施加0.02、0.4、1.0、2.2、3.3和4.3MHz的超声波，研究了超声波对这些藻类的破碎效率。结果表明，藻类数量的减少与频率相关，对于纤细角毛藻、钙板角毛藻和微拟球藻，分别在2.2、3.3和4.3MHz时达到最高破碎效率。文献综述表明，超声波破碎藻类需要空化作用，而非超声处理的直接作用，并且化学作用可能不是藻类细胞破碎的主要机制。考虑弹性特性通过壳模型估算的机械共振频率表明，每种藻类的合适破碎频率与细胞的机械特性相关。综上所述，我们认为超声处理的物理作用是藻类破碎的原因。
