Zhang Mei-Fang, Qin Yuan-Hang, Ma Jia-Yu, Yang Li, Wu Zai-Kun, Wang Tie-Lin, Wang Wei-Guo, Wang Cun-Wen
Key Laboratory of Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China.
Key Laboratory of Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Wuhan 430073, China.
Ultrason Sonochem. 2016 Jul;31:404-8. doi: 10.1016/j.ultsonch.2016.01.027. Epub 2016 Jan 26.
In this study, the combined use of Fenton reagent and ultrasound to the pretreatment of microcrystalline cellulose (MCC) for subsequent enzyme hydrolysis was investigated. The morphological analysis showed that the aspect ratio of MCC was greatly reduced after pretreatment. The X-ray diffraction (XRD) and degree of polymerization (DP) analyses showed that Fenton reagent was more efficient in decreasing the crystallinity of MCC while ultrasound was more efficient in decreasing the DP of MCC. The combination of Fenton reaction and ultrasound, which produced the lowest crystallinity (84.8 ± 0.2%) and DP (124.7 ± 0.6) of MCC and the highest yield of reducing sugar (22.9 ± 0.3 g/100 g), provides a promising pretreatment process for MCC depolymerization.
在本研究中，考察了芬顿试剂与超声联合用于微晶纤维素（MCC）预处理以进行后续酶水解的情况。形态分析表明，预处理后MCC的长径比大幅降低。X射线衍射（XRD）和聚合度（DP）分析表明，芬顿试剂在降低MCC结晶度方面更有效，而超声在降低MCC的DP方面更有效。芬顿反应与超声的联合使用产生了MCC的最低结晶度（84.8±0.2%）和DP（124.7±0.6）以及最高还原糖产率（22.9±0.3 g/100 g），为MCC解聚提供了一种有前景的预处理工艺。
