Ogasawara Shinzi
Creative Research Institution Sousei (CRIS), Hokkaido University, Kita 21 Nishi 10, Kita-ku, Sapporo, Hokkaido, 001-0021, Japan.
Methods Mol Biol. 2016;1408:55-66. doi: 10.1007/978-1-4939-3512-3_4.
Several methods for controlling gene expression by light illumination have been reported. Most of these methods control transcription by regulating the interaction between DNA and transcription factors. The use of a photolabile protecting compound (cage compound) is another promising approach for controlling gene expression, although typically in an irreversible manner. We here describe a new approach for reversibly controlling translation using a photoresponsive 8-styryl cap (8ST-cap) that can be reversibly isomerized by illumination with light of a specific wavelength.
已经报道了几种通过光照来控制基因表达的方法。这些方法大多通过调节DNA与转录因子之间的相互作用来控制转录。使用光不稳定保护化合物（笼形化合物）是另一种控制基因表达的有前景的方法，尽管通常是以不可逆的方式。我们在此描述一种使用光响应性8-苯乙烯基帽（8ST-帽）来可逆地控制翻译的新方法，该帽可通过特定波长的光照进行可逆异构化。
