伊朗城市和生活污水中分离出的十二指肠贾第鞭毛虫分离株的亚型分析。
Subtype analysis of Giardia duodenalis isolates from municipal and domestic raw wastewaters in Iran.
作者信息
Hatam-Nahavandi Kareem, Mohebali Mehdi, Mahvi Amir-Hossein, Keshavarz Hossein, Mirjalali Hamed, Rezaei Sasan, Meamar Ahmad-Reza, Rezaeian Mostafa
机构信息
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Environ Sci Pollut Res Int. 2017 May;24(14):12740-12747. doi: 10.1007/s11356-016-6316-y. Epub 2016 Mar 11.
A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses in Tehran, Iran, were assessed for the presence of the Giardia cysts using immunofluorescence with monoclonal antibodies. To characterize the cysts at the molecular level, the three genetic loci were amplified and sequenced. The assemblages A (37.5 %) and E (58.3 %) were detected in livestock wastewater samples. Assemblage A, which is composed of only G. duodenalis genotype, was detected in 100 % of urban wastewater samples. The subassemblages A2, A3, A-I, A-II, and E3 were identified with β-giardin, triose phosphate isomerase, and glutamate dehydrogenase genes. This study is the first to report on G. duodenalis genotypes in aquatic environmental samples in Iran.
从伊朗德黑兰的三个城市污水处理厂和两个屠宰场采集了总共54份未经处理的废水样本，采用单克隆抗体免疫荧光法评估贾第虫囊肿的存在情况。为了在分子水平上对囊肿进行表征，对三个基因位点进行了扩增和测序。在牲畜废水样本中检测到组合A（37.5%）和组合E（58.3%）。仅由十二指肠贾第虫基因型组成的组合A在100%的城市废水样本中被检测到。通过β-贾第蛋白、磷酸丙糖异构酶和谷氨酸脱氢酶基因鉴定出了亚组合A2、A3、A-I、A-II和E3。本研究首次报道了伊朗水生环境样本中的十二指肠贾第虫基因型。
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