Department of Ecosystem Analysis, Institute for Environmental Research, ABBt-Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany.
Department G3: Biochemistry, Ecotoxicology, Federal Institute of Hydrology (BfG), Am Mainzer Tor 1, 56068, Koblenz, Germany.
Environ Sci Pollut Res Int. 2016 Jun;23(12):12060-74. doi: 10.1007/s11356-016-6377-y. Epub 2016 Mar 11.
To examine the uptake of dioxin-like compounds (DLCs), common roaches (Rutilus rutilus) were exposed for 28 days to differently contaminated sediments from two major European rivers in a purpose-built facility. Dietary transfer of DLCs was investigated by exposing fish to sediments inoculated or non-inoculated with black worms (Lumbriculus variegatus). Dioxin-like polychlorinated biphenyls (DL-PCBs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), measured via high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) in sediments and whole fish, were used to calculate toxicity equivalent quotients (TEQs). TEQs were compared with biological toxicity equivalent quotients (BEQs) determined via the 7-ethoxyresorufin-O-deethylase (EROD) assay, performed with mammalian (H4IIE) and fish (RTL-W1) liver cell lines. TEQs and BEQs indicated an uptake of sediment-borne DLCs by roach, which was independent of sediment contamination levels, but rather reflected sediment-specific characteristics. For most sediment treatments, DLC uptake did not increase with time. Highest congener-specific uptake (DL-PCB 123) was 10-fold compared to control. Exposure to worm-inoculated sediment of highest overall DLC contamination caused a 2-fold (TEQ and H4IIE BEQ) greater uptake of DLCs by fish compared to the respective non-inoculated treatment. H4IIE cells showed the greatest sensitivity (0.37 ± 0.25 pM TCDD) and the strongest correlation with TEQs (r (2) = 0.79), hence, they seem to be best suited for DLC screening of sediments and biota, amended by compound-specific instrumental analysis if required.
为了研究二恶英样化合物（DLCs）的摄取情况，研究人员在一个专门设计的设施中，用来自两条欧洲主要河流的不同污染沉积物对普通河鳜（Rutilus rutilus）进行了 28 天的暴露实验。通过将鱼暴露于接种或未接种黑蚯蚓（Lumbriculus variegatus）的沉积物中，研究了 DLCs 的饮食传递。使用高分辨率气相色谱/高分辨率质谱法（HRGC/HRMS）在沉积物和整条鱼中测量二恶英样多氯联苯（DL-PCBs）、多氯二苯并对二恶英和二苯并呋喃（PCDD/Fs），以计算毒性等效系数（TEQs）。TEQs 与通过哺乳动物（H4IIE）和鱼类（RTL-W1）肝细胞系进行的 7-乙氧基resorufin-O-脱乙基酶（EROD）测定确定的生物毒性等效系数（BEQs）进行了比较。TEQs 和 BEQs 表明，鳜鱼摄取了来自沉积物的 DLCs，这种摄取与沉积物的污染水平无关，而是反映了沉积物的特定特征。对于大多数沉积物处理，DLC 的摄取并没有随时间的增加而增加。最高的同类物特异性摄取（DL-PCB 123）是对照的 10 倍。与各自的未接种处理相比，暴露于最高总体 DLC 污染的蚯蚓接种沉积物会导致鱼类对 DLCs 的摄取增加两倍（TEQ 和 H4IIE BEQ）。H4IIE 细胞表现出最大的敏感性（0.37±0.25 pM TCDD）和与 TEQs 的最强相关性（r (2) = 0.79），因此，如果需要，它们似乎最适合用于沉积物和生物群中 DLC 的筛选，并通过化合物特异性仪器分析进行修正。
