Gales Grégoire, Tsesmetzis Nicolas, Neria Isabel, Alazard Didier, Coulon Stéphanie, Lomans Bart P, Morin Dominique, Ollivier Bernard, Borgomano Jean, Joulian Catherine
Aix-Marseille Université, CEREGE, Centre St Charles, Case 67, 3 Place Victor Hugo, 13331 Marseille, France.
Aix-Marseille Université, Université du Sud Toulon-Var, CNRS/INSU, IRD, MIO, UM 110, 13288, Marseille, cedex 09, France.
Sci Rep. 2016 Mar 11;6:22960. doi: 10.1038/srep22960.
Microbiology of a hypersaline oil reservoir located in Central Africa was investigated with molecular and culture methods applied to preserved core samples. Here we show that the community structure was partially acquired during sedimentation, as many prokaryotic 16S rRNA gene sequences retrieved from the extracted DNA are phylogenetically related to actual Archaea inhabiting surface evaporitic environments, similar to the Cretaceous sediment paleoenvironment. Results are discussed in term of microorganisms and/or DNA preservation in such hypersaline and Mg-rich solutions. High salt concentrations together with anaerobic conditions could have preserved microbial/molecular diversity originating from the ancient sediment basin wherein organic matter was deposited.
运用分子和培养方法对保存的岩心样本进行研究，以调查位于中非的一个超盐油藏的微生物群落。我们在此表明，群落结构部分是在沉积过程中形成的，因为从提取的DNA中检索到的许多原核生物16S rRNA基因序列在系统发育上与栖息于地表蒸发环境的实际古菌相关，类似于白垩纪沉积古环境。从微生物和/或DNA在这种高盐且富含镁的溶液中的保存情况对结果进行了讨论。高盐浓度和厌氧条件可能保存了源自古代沉积盆地（其中沉积了有机物）的微生物/分子多样性。
