人角膜缘间充质干细胞表达ABCB5，且能在羊膜上生长。
Human limbal mesenchymal stem cells express ABCB5 and can grow on amniotic membrane.
作者信息
Shaharuddin Bakiah, Osei-Bempong Charles, Ahmad Sajjad, Rooney Paul, Ali Simi, Oldershaw Rachel, Meeson Annette
机构信息
Institute of Genetic Medicine, Faculty of Medical Sciences, Newcastle University, Newcastle Upon-Tyne, NE1 3BZ, UK.
Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200 Pulau Pinang, Malaysia.
出版信息
Regen Med. 2016 Apr;11(3):273-86. doi: 10.2217/rme-2016-0009. Epub 2016 Mar 11.
AIM
To isolate and characterize limbal mesenchymal stem cells (LMSCs) from human corneoscleral rings.
MATERIALS & METHODS: Cells were isolated from corneoscleral rings and cultured in a mesenchymal stem cell (MSC)-selective media and examined for differentiation, phenotyping and characterization.
RESULTS
LMSCs were capable of trilineage differentiation, adhered to tissue culture plastic, expressed HLA class I and cell surface antigens associated with human MSC while having no/low expression of HLA class II and negative hematopoietic lineage markers. They were capable for CXCL12-mediated cellular migration. LMSCs adhered, proliferated on amniotic membrane and expressed the common putative limbal stem cell markers.
CONCLUSION
Limbal-derived MSC exhibited plasticity, could maintain limbal markers expression and demonstrated viable growth on amniotic membrane.
目的
从人角膜缘巩膜环中分离并鉴定角膜缘间充质干细胞（LMSCs）。
材料与方法
从角膜缘巩膜环中分离细胞，在间充质干细胞（MSC）选择性培养基中培养，并对其分化、表型和特性进行检测。
结果
LMSCs能够三系分化，贴附于组织培养塑料上，表达I类人类白细胞抗原（HLA）和与人MSC相关的细胞表面抗原，而II类HLA表达无/低，造血谱系标志物为阴性。它们能够进行CXCL12介导的细胞迁移。LMSCs能在羊膜上贴附、增殖并表达常见的假定角膜缘干细胞标志物。
结论
源自角膜缘的MSC表现出可塑性，能够维持角膜缘标志物表达，并在羊膜上显示出良好的生长状态。
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