利用产油酵母粘红酵母将造纸厂污泥转化为中性脂类物质生产生物柴油。
Converting paper mill sludge into neutral lipids by oleaginous yeast Cryptococcus vishniaccii for biodiesel production.
机构信息
Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee (IIT R), Saharanpur Campus, Saharanpur 247001, India.
Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
出版信息
Bioresour Technol. 2016 Aug;213:96-102. doi: 10.1016/j.biortech.2016.02.105. Epub 2016 Mar 3.
Paper mill sludge (PMS) was assessed as cheap renewable lignocellulosic biomass for lipid production by the oleaginous yeast Cryptococcus vishniaccii (MTCC 232). The sonicated paper mill sludge extract (PMSE) exhibited enhanced lipid yield and lipid content 7.8±0.57g/l, 53.40% in comparison to 5.5±0.8g/l, 40.44% glucose synthetic medium, respectively. The accumulated triglycerides (TAG) inside the lipid droplets (LDs) were converted to biodiesel by transesterification and thoroughly characterized using GC-MS technique. The fatty acid methyl ester (FAME) profile obtained reveals elevated content of oleic acid followed by palmitic acid, linoleic acid and stearic acid with improved oxidative stability related to biodiesel quality.
造纸厂污泥（PMS）被评估为一种廉价的可再生木质纤维素生物质，可用于产油酵母粘红酵母（MTCC 232）的脂质生产。与葡萄糖合成培养基（5.5±0.8g/l，40.44%）相比，超声处理后的造纸厂污泥提取物（PMSE）显示出更高的脂质产率和脂质含量（7.8±0.57g/l，53.40%）。通过酯交换反应将脂质滴（LDs）内的积累的三酰基甘油(TAG)转化为生物柴油，并使用 GC-MS 技术进行了全面表征。获得的脂肪酸甲酯（FAME）谱表明，油酸含量升高，其次是棕榈酸、亚油酸和硬脂酸，与生物柴油质量相关的氧化稳定性得到改善。
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