利用落地花生壳在新型产油酵母 Cryptococcus psychrotolerans IITRFD 中促进三酰基甘油积累以提高生物柴油产量。
Fostering triacylglycerol accumulation in novel oleaginous yeast Cryptococcus psychrotolerans IITRFD utilizing groundnut shell for improved biodiesel production.
机构信息
Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee (IIT R), Saharanpur Campus, Saharanpur 247001, India.
Department of Biotechnology, Indian Institute of Technology Roorkee (IIT R), Roorkee, Uttarakhand 247667, India.
出版信息
Bioresour Technol. 2017 Oct;242:113-120. doi: 10.1016/j.biortech.2017.04.001. Epub 2017 Apr 4.
The investigation was carried out to examine the potential of triacylglycerol (TAG) accumulation by novel oleaginous yeast isolate Cryptococcus psychrotolerans IITRFD on utilizing groundnut shell acid hydrolysate (GSH) as cost-effective medium. The maximum biomass productivity and lipid productivity of 0.095±0.008g/L/h and 0.044±0.005g/L/h, respectively with lipid content 46% was recorded on GSH. Fatty acid methyl ester (FAME) profile obtained by GC-MS analysis revealed oleic acid (37.8%), palmitic (29.4%) and linoleic (32.8%) as major fatty acids representing balance between oxidative stability (OS) and cold flow filter properties (CFFP) for improved biodiesel quality. The biodiesel property calculated were correlated well with the fuel standards limits of ASTM D6751, EN 14214 and IS 15607. The present findings raise the possibility of using agricultural waste groundnut shell as a substrate for production of biodiesel by novel oleaginous yeast isolate C. psychrotolerans IITRFD.
本研究旨在考察新型耐冷油脂酵母Cryptococcus psychrotolerans IITRFD 利用花生壳酸水解物（GSH）作为经济型培养基积累三酰基甘油（TAG）的潜力。在 GSH 上，分别记录到最大生物量生产力和脂质生产力为 0.095±0.008g/L/h 和 0.044±0.005g/L/h，脂质含量为 46%。GC-MS 分析得到的脂肪酸甲酯（FAME）图谱显示，油酸（37.8%）、棕榈酸（29.4%）和亚油酸（32.8%）为主要脂肪酸，这代表了氧化稳定性（OS）和冷滤堵塞点（CFFP）之间的平衡，有利于提高生物柴油的质量。计算出的生物柴油特性与 ASTM D6751、EN 14214 和 IS 15607 的燃料标准限值密切相关。本研究结果表明，利用新型耐冷油脂酵母 C. psychrotolerans IITRFD 利用农业废弃物花生壳作为生物柴油生产的底物具有一定的可行性。
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