Department of Chemistry and Biodynamics of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10 St., 10-748 Olsztyn, Poland.
Department of Immunology and Food Microbiology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Food Funct. 2016 Apr;7(4):1950-8. doi: 10.1039/c6fo00140h.
Compromised intestinal calcium absorption affecting a deterioration of bone state is a sign of coeliac disease. Experimental calcium-fortified gluten-free bread (GFB) of improved calcium bioavailability could increase calcium content in the diets of coeliac disease patients, allowing them to obtain the amount of calcium they need for therapeutic use. Prebiotics, including inulin-type fructans (IFs) have a beneficial effect on calcium bioavailability. In the present study, the in vitro model composed of the intestinal-like Caco-2 cells and the human intestinal bacteria (Lactobacillus, Enterococcus and Enterobacteriaceae) were used to analyse the effect of inulin and fructooligosaccharide (FOS) of different chain lengths, on calcium uptake and absorption from experimental GFB. Analysed IFs, especially short-chain FOS, significantly (p < 0.05) increased cellular calcium uptake from GFB digest and stimulated the intestinal bacteria applied in the cultures to the intensive synthesis of organic acids. In particular, the concentration of butyric, valeric and lactic acids increased significantly. Similarly, in the calcium absorption experiment, IFs increased the cellular calcium retention but concomitantly reduced its content in basolateral filtrates. The results obtained suggest that the applied IFs affected differentially calcium uptake and absorption from the experimental calcium-enriched GFB, therefore a further study is needed to assess whether these observations made in vitro contribute to IF effects on calcium absorption from experimental GFB in vivo.
肠道钙吸收受损会导致骨状态恶化，这是乳糜泻的一个迹象。经过改良、可提高钙生物利用度的钙强化无麸质面包（GFB），可以增加乳糜泻患者饮食中的钙含量，使他们能够获得治疗所需的钙量。包括菊粉型果聚糖（IFs）在内的益生元对钙生物利用度有有益影响。在本研究中，使用由肠样 Caco-2 细胞和人类肠道细菌（乳杆菌、肠球菌和肠杆菌科）组成的体外模型，分析了不同链长的菊糖和低聚果糖（FOS）对从实验性 GFB 中摄取和吸收钙的影响。分析的 IFs，特别是短链 FOS，可显著（p < 0.05）增加 GFB 消化物中的细胞钙摄取，并刺激应用于培养物中的肠道细菌强烈合成有机酸。特别是丁酸、戊酸和乳酸的浓度显著增加。同样，在钙吸收实验中，IFs增加了细胞内钙的保留，但同时降低了基底外侧滤液中的钙含量。研究结果表明，所应用的 IFs 可不同程度地影响从实验性富钙 GFB 中摄取和吸收钙，因此需要进一步研究，以评估这些在体外观察到的结果是否有助于 IF 对实验性 GFB 中钙吸收的影响。
