重组黄尾鰤（Seriola lalandi）促卵泡激素的体外和体内生物学活性
In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone.
作者信息
Sanchís-Benlloch Pablo J, Nocillado Josephine, Ladisa Claudia, Aizen Joseph, Miller Adam, Shpilman Michal, Levavi-Sivan Berta, Ventura Tomer, Elizur Abigail
机构信息
Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Queensland, Australia.
Clean Seas Tuna Ltd, Port Lincoln, SA, Australia.
出版信息
Gen Comp Endocrinol. 2017 Jan 15;241:41-49. doi: 10.1016/j.ygcen.2016.03.001. Epub 2016 Mar 7.
Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.
具有生物活性的重组黄尾鰤促卵泡激素（rytkFsh）在毕赤酵母中产生，其生物活性通过体外和体内生物测定得以证实。用重组激素孵育卵巢和睾丸片段分别刺激了雌二醇（E）和11-酮睾酮（11-KT）的分泌。在未成熟雌性黄尾鰤体内进行的试验导致血浆E水平显著升高以及卵母细胞发育。在青春期早期的雄性中，观察到精子发生提前，然而，当给予rytkFsh时，血浆11-KT水平降低。
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