Yadawa Arun Kumar, Chaturvedi Chandra Mohini
Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Department of Zoology, Banaras Hindu University, Varanasi 221005, India.
Gen Comp Endocrinol. 2016 Dec 1;239:13-20. doi: 10.1016/j.ygcen.2016.03.005. Epub 2016 Mar 7.
Neurohypophyseal hormone, arginine vasopressin (AVP), in addition to acting as antidiuretic hormone is also considered to be stress hormone like hypothalamic corticotropin-releasing hormone (CRH). Present study was designed to investigate the relative response of these stress hormones during water and food deprivation. In this study, male laboratory mice of Swiss strain were divided in 5 groups, control - provided water and food ad libitum, two experimental groups water deprived for 2 and 4days respectively (WD2 and WD4) and another two groups food deprived for 2 and 4days respectively (FD2 and FD4). Results indicate an increased expression of AVP mRNA as well as peptide in the hypothalamus of WD2 mice and the expression was further upregulated after 4days of water deprivation but the expression of CRH remained unchanged compare to their respective controls. On the other hand no change was observed in the expression of hypothalamic AVP mRNA while AVP peptide increased significantly in FD2 and FD4 mice compare to control. Further, the expression of CRH mRNA although increased in hypothalamus of both FD2 and FD4 mice, the immunofluorescent staining shows decreased expression of CRH in PVN of food deprived mice. Based on these findings it is concluded that since during osmotic stress only AVP expression is upregulated but during metabolic stress i.e. food deprivation transcription and translation of both the stress hormones are differentially regulated. Further, it is suggested that role of AVP and CRH may be stress specific.
神经垂体激素精氨酸加压素（AVP），除了作为抗利尿激素外，还被认为是一种应激激素，类似于下丘脑促肾上腺皮质激素释放激素（CRH）。本研究旨在调查在缺水和禁食期间这些应激激素的相对反应。在本研究中，将瑞士品系的雄性实验小鼠分为5组，对照组自由饮水和进食，两个实验组分别缺水2天和4天（WD2和WD4），另外两个组分别禁食2天和4天（FD2和FD4）。结果表明，WD2小鼠下丘脑AVP mRNA及其肽的表达增加，缺水4天后表达进一步上调，但与各自对照组相比，CRH的表达保持不变。另一方面，与对照组相比，FD2和FD4小鼠下丘脑AVP mRNA的表达没有变化，而AVP肽显著增加。此外，虽然FD2和FD4小鼠下丘脑CRH mRNA的表达均增加，但免疫荧光染色显示禁食小鼠PVN中CRH的表达降低。基于这些发现得出结论，在渗透应激期间只有AVP的表达上调，但在代谢应激即禁食期间，两种应激激素的转录和翻译受到不同调节。此外，提示AVP和CRH的作用可能具有应激特异性。
