Carter Lindsay G, Ngo Tenlep Sara Y, Woollett Laura A, Pearson Kevin J
Pharmacology and Nutritional Sciences, College of Medicine, University of Kentucky, 900 South Limestone, Lexington, KY 40536-0200, USA.
Department of Pathology and Laboratory Medicine, Medical Diseases Institute, University of Cincinnati Medical School, 2180 East Galbraith Rd, Cincinnati, OH 45237-0507, USA.
J Diabetes Metab. 2015 Dec;6(12). doi: 10.4172/2155-156.1000634. Epub 2015 Dec 30.
Physical activity has been suggested as a non-pharmacological intervention that can be used to improve glucose homeostasis in women with gestational diabetes mellitus. The purpose of this study was to determine the effects of voluntary exercise on glucose tolerance and body composition in pregnant high fat diet fed mice.
Female mice were put on a standard diet or high fat diet for two weeks. The mice were then split into 4 groups; control standard diet fed, exercise standard diet fed, control high fat diet fed, and exercise high fat diet fed. Exercise mice had voluntary access to a running wheel in their home cage one week prior to mating, during mating, and throughout pregnancy. Glucose tolerance and body composition were measured during pregnancy. Akt levels were quantified in skeletal muscle and adipose tissue isolated from saline or insulin injected pregnant dams as a marker for insulin signaling.
Consumption of the high fat diet led to significantly increased body weight, fat mass, and impaired glucose tolerance in control mice. However, voluntary running in the high fat diet fed dams significantly reduced weight gain and fat mass and ultimately improved glucose tolerance compared to control high fat diet fed dams. Further, body weight, fat mass, and glucose disposal in exercise high fat diet dams were indistinguishable from control dams fed the standard diet. High fat diet fed exercise dams also had significantly increased insulin stimulated phosphorylated Akt expression in adipose tissue, but not skeletal muscle, compared to control dams on high fat diet.
The use of voluntary exercise improves glucose homeostasis and body composition in pregnant female mice. Thus, future studies could investigate potential long-term health benefits in offspring born to obese exercising dams.
体育活动被认为是一种非药物干预措施，可用于改善妊娠期糖尿病女性的葡萄糖稳态。本研究的目的是确定自愿运动对高脂饮食喂养的怀孕小鼠的葡萄糖耐量和身体成分的影响。
将雌性小鼠置于标准饮食或高脂饮食中两周。然后将小鼠分为4组；对照标准饮食喂养组、运动标准饮食喂养组、对照高脂饮食喂养组和运动高脂饮食喂养组。运动小鼠在交配前一周、交配期间和整个怀孕期间可在其笼舍中自由使用跑步轮。在怀孕期间测量葡萄糖耐量和身体成分。对从注射生理盐水或胰岛素的怀孕母鼠分离的骨骼肌和脂肪组织中的Akt水平进行定量，作为胰岛素信号的标志物。
高脂饮食导致对照小鼠体重、脂肪量显著增加，葡萄糖耐量受损。然而，与对照高脂饮食喂养的母鼠相比，高脂饮食喂养的母鼠自愿跑步显著降低了体重增加和脂肪量，并最终改善了葡萄糖耐量。此外，运动高脂饮食母鼠的体重、脂肪量和葡萄糖代谢与标准饮食喂养的对照母鼠没有区别。与高脂饮食的对照母鼠相比，高脂饮食喂养的运动母鼠在脂肪组织中胰岛素刺激的磷酸化Akt表达也显著增加，但在骨骼肌中没有。
自愿运动可改善怀孕雌性小鼠的葡萄糖稳态和身体成分。因此，未来的研究可以调查肥胖运动母鼠所产后代潜在的长期健康益处。
