非肥胖糖尿病（NOD）小鼠和正常（NOR）小鼠血液白细胞亚群的纵向频率存在差异，但不能预测NOD小鼠的糖尿病发生。

Longitudinal Frequencies of Blood Leukocyte Subpopulations Differ between NOD and NOR Mice but Do Not Predict Diabetes in NOD Mice.

作者信息

Telieps Tanja, Köhler Meike, Treise Irina, Foertsch Katharina, Adler Thure, Busch Dirk H, Hrabě de Angelis Martin, Verschoor Admar, Adler Kerstin, Bonifacio Ezio, Ziegler Anette-Gabriele

机构信息

Institute for Diabetes and Obesity, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.

Institute of Diabetes Research, Helmholtz Zentrum München and Forschergruppe Diabetes, Klinikum rechts der Isar, Technische Universität München, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.

出版信息

J Diabetes Res. 2016;2016:4208156. doi: 10.1155/2016/4208156. Epub 2016 Feb 4.

DOI:10.1155/2016/4208156

PMID:26966692

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4757706/

Abstract

Immune phenotyping provides insight into disease pathogenesis and prognostic markers. Trajectories from age of 4 to 36 weeks were modeled for insulin autoantibodies and for leukocyte subpopulations in peripheral blood from female NOD (n = 58) and NOR (n = 22) mice. NOD mice had higher trajectories of insulin autoantibodies, CD4(+) and CD8(+) T lymphocytes, B lymphocytes, IgD(+)IgM(-) B lymphocytes, and NK cells and lower trajectories of CD4(+)CD25(+) T lymphocytes, IgM(+) B lymphocytes, granulocytes, and monocytes than NOR mice (all p < 0.001). Of these, only the increased IAA trajectory was observed in NOD mice that developed diabetes as compared to NOD mice that remained diabetes-free. Therefore, the profound differences in peripheral blood leukocyte proportions observed between the diabetes-prone NOD mice and the diabetes-resistant mice do not explain the variation in diabetes development within NOD mice and do not provide markers for diabetes prediction in this model.

摘要

免疫表型分析有助于深入了解疾病的发病机制和预后标志物。对雌性非肥胖糖尿病（NOD，n = 58）和正常（NOR，n = 22）小鼠外周血中胰岛素自身抗体和白细胞亚群从4周龄到36周龄的变化轨迹进行了建模。与NOR小鼠相比，NOD小鼠的胰岛素自身抗体、CD4(+)和CD8(+) T淋巴细胞、B淋巴细胞、IgD(+)IgM(-) B淋巴细胞和自然杀伤细胞的变化轨迹更高，而CD4(+)CD25(+) T淋巴细胞、IgM(+) B淋巴细胞、粒细胞和单核细胞的变化轨迹更低（所有p < 0.001）。其中，与未患糖尿病的NOD小鼠相比，在患糖尿病的NOD小鼠中仅观察到胰岛素自身抗体轨迹增加。因此，在易患糖尿病的NOD小鼠和抗糖尿病小鼠之间观察到的外周血白细胞比例的显著差异并不能解释NOD小鼠中糖尿病发展的差异，也不能为该模型中的糖尿病预测提供标志物。