Rodriguez-Rivera Veronica, Weidner John W, Yost Michael J
Department of Surgery - Division of General Surgery, Medical University of South Carolina.
Department of Chemical Engineering, University of South Carolina.
J Vis Exp. 2016 Feb 12(108):53578. doi: 10.3791/53578.
Tissue scaffolds play a crucial role in the tissue regeneration process. The ideal scaffold must fulfill several requirements such as having proper composition, targeted modulus, and well-defined architectural features. Biomaterials that recapitulate the intrinsic architecture of in vivo tissue are vital for studying diseases as well as to facilitate the regeneration of lost and malformed soft tissue. A novel biofabrication technique was developed which combines state of the art imaging, three-dimensional (3D) printing, and selective enzymatic activity to create a new generation of biomaterials for research and clinical application. The developed material, Bovine Serum Albumin rubber, is reaction injected into a mold that upholds specific geometrical features. This sacrificial material allows the adequate transfer of architectural features to a natural scaffold material. The prototype consists of a 3D collagen scaffold with 4 and 3 mm channels that represent a branched architecture. This paper emphasizes the use of this biofabrication technique for the generation of natural constructs. This protocol utilizes a computer-aided software (CAD) to manufacture a solid mold which will be reaction injected with BSA rubber followed by the enzymatic digestion of the rubber, leaving its architectural features within the scaffold material.
组织支架在组织再生过程中起着至关重要的作用。理想的支架必须满足几个要求，如具有合适的成分、目标模量和明确的结构特征。能够重现体内组织固有结构的生物材料对于研究疾病以及促进受损和畸形软组织的再生至关重要。一种新的生物制造技术被开发出来，它结合了先进的成像技术、三维（3D）打印技术和选择性酶活性，以制造用于研究和临床应用的新一代生物材料。所开发的材料，牛血清白蛋白橡胶，被反应注射到具有特定几何特征的模具中。这种牺牲性材料允许将结构特征充分转移到天然支架材料上。该原型由一个具有4毫米和3毫米通道的3D胶原蛋白支架组成，这些通道代表了一种分支结构。本文强调了这种生物制造技术在生成天然构建体方面的应用。该方案利用计算机辅助软件（CAD）制造一个固体模具，将牛血清白蛋白橡胶反应注射到该模具中，随后对橡胶进行酶消化，使其结构特征保留在支架材料内。
