Shirakawa Makoto, Ueda Haruko, Shimada Tomoo, Hara-Nishimura Ikuko
a Graduate School of Science, Kyoto University , Kyoto 606-8502 , Japan.
Plant Signal Behav. 2016;11(4):e1150403. doi: 10.1080/15592324.2016.1150403.
Myrosin cells accumulate myrosinases in their vacuoles to catalyze the production of toxic compounds when tissues are damaged by herbivores. Myrosin cells are positioned adjacent to the abaxial side of the vasculature but their origin is unclear. To determine whether the myrosin cells are differentiated from vascular precursor cells, we generated a transgenic Arabidopsis line that expressed a myrosin cell reporter together with one of 3 vascular precursor cell reporters. The myrosin-positive cells were discontinuously distributed while the vascular precursor-positive cells were continuously distributed. The fluorescent signals of the myosin and vascular reporters did not overlap. Furthermore, the shape of myrosin-positive cells was different from the shape of vascular precursor-positive cells. These results indicate that myosin cells develop independently of the vasculature.
芥子酶细胞在其液泡中积累芥子酶，以便在组织被食草动物损伤时催化有毒化合物的产生。芥子酶细胞位于脉管系统远轴侧附近，但其起源尚不清楚。为了确定芥子酶细胞是否由维管前体细胞分化而来，我们构建了一个转基因拟南芥品系，该品系表达一个芥子酶细胞报告基因以及3个维管前体细胞报告基因之一。芥子酶阳性细胞呈间断分布，而维管前体阳性细胞呈连续分布。芥子酶和维管报告基因的荧光信号不重叠。此外，芥子酶阳性细胞的形状与维管前体阳性细胞的形状不同。这些结果表明，芥子酶细胞独立于脉管系统发育。
