Vandivort Tyler C, An Dowon, Parks William C
Department of Environmental and Occupational Health Sciences, University of Washington; Department of Medicine, Cedars-Sinai Medical Center;
Division of Pulmonary and Critical Care Medicine, University of Washington.
J Vis Exp. 2016 Feb 22(108):53771. doi: 10.3791/53771.
Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity is a commonly used model to study both acute lung injury and fibrosis, and multiple methods have been developed for administering bleomycin (and other toxic agents) into the lungs. However, many of these approaches, such as transtracheal instillation, have inherent drawbacks, including the need for strong anesthetics and survival surgery. This paper reports a quick, reproducible method of intratracheal intubation that involves mild inhaled anesthesia, visualization of the trachea, and the use of a surrogate spirometer to confirm exposure. As a proof of concept, 8-12 week old C57BL/6 mice were administered either 2.0 U/kg of bleomycin or an equivalent volume of PBS, and both damage and fibrotic endpoints were measured post-exposure. This procedure allows researchers to treat a large cohort of mice in a relatively short period with little expense and minimal post-procedure care.
尽管存在一些解剖学和生理学差异，但小鼠模型仍然是研究人类肺部疾病的重要工具。博来霉素毒性是用于研究急性肺损伤和纤维化的常用模型，并且已经开发了多种将博来霉素（和其他有毒剂）注入肺部的方法。然而，这些方法中的许多方法，如经气管滴注，都有其固有的缺点，包括需要强效麻醉剂和生存手术。本文报道了一种快速、可重复的气管插管方法，该方法涉及轻度吸入麻醉、气管可视化以及使用替代肺活量计来确认暴露情况。作为概念验证，给8至12周龄的C57BL/6小鼠注射2.0 U/kg的博来霉素或等量体积的PBS，并在暴露后测量损伤和纤维化终点。该程序使研究人员能够在相对较短的时间内以低成本和最少的术后护理治疗大量小鼠。
