Hsu Dur-Zong, Jou I-Ming
Department of Orthopedics, National Cheng Kung University Hospital, Tainan, Taiwan.
Department of Orthopedics, College of Medicine, National Cheng Kung University, 138 Sheng-Li Road, Tainan, 704, Taiwan.
J Mater Sci Mater Med. 2016 May;27(5):84. doi: 10.1007/s10856-016-5689-2. Epub 2016 Mar 11.
Epidural fibrosis, an inevitable part of the postoperative healing process, is one of the important causes of failed back surgery syndrome after spinal surgery. The aim of this study was to examine the inhibitory effect of a novel material 1,4-butanediol diglycidyl ether-cross-linked hyaluronan (cHA) on fibrosis in primary tenocytes. cHA inhibited migration, cell proliferation, and suppressed the expression of fibronectin, but not transforming growth factor-β, in primary tenocytes. cHA significantly increased matrix metalloproteinase-3 but decreased collagen-1 and microtubule-associated protein light chain 3-II expression in a dose-dependent manner compared with control groups. We therefore concluded that suppressing autophagy activity may be involved in the anti-fibrotic effect of cHA in primary tenocytes. Further, cHA may have the potential for preventing epidural fibrosis and subsequent failed back syndrome in patients with laminectomy in the future.
硬膜外纤维化是术后愈合过程中不可避免的一部分，是脊柱手术后腰椎手术失败综合征的重要原因之一。本研究的目的是检测新型材料1,4 - 丁二醇二缩水甘油醚交联透明质酸（cHA）对原代肌腱细胞纤维化的抑制作用。cHA抑制原代肌腱细胞的迁移、细胞增殖，并抑制纤连蛋白的表达，但不影响转化生长因子-β的表达。与对照组相比，cHA以剂量依赖性方式显著增加基质金属蛋白酶-3的表达，但降低胶原蛋白-1和微管相关蛋白轻链3-II的表达。因此，我们得出结论，抑制自噬活性可能参与了cHA对原代肌腱细胞的抗纤维化作用。此外，cHA未来可能具有预防椎板切除术后患者硬膜外纤维化及随后的腰椎手术失败综合征的潜力。
