Wegiel J, Medyńska E, Dziedziak W, Szirkowiec-Gmurczyk W, Dymecki J
Neuropatol Pol. 1989;27(2):279-94.
The aim of the studies was to elaborate technical procedure of material preparation for quantitative studies and determination of correction coefficients of morphometric parameters in dependence on histological procedures. Moreover, volume changes of the rat substantia nigra and striatum (structures degenerating in Parkinson's disease) during the first half year of life were estimated. As fixatives 4 and 8% formaldehyde in 4 and 20 degrees C were used. Irrespective of applied variant of fixation formaldehyde produced during first 48 hrs rapid increase of the brain weight and volume up to 52% of that of the fresh brain, which was followed by slow decrease of brain weight of about 1-3%/24 hrs. The most pronounced changes caused 4% formaldehyde in 20 degrees C. The best conditions for morphometric studies were obtained with the use of 8% formaldehyde in 20 degrees C. Dehydration in ethyl alcohol produced violent decrease of brain volume and weight (from 32% up to 39% of the fresh brain weight). Clearing in methyl bensoesane increases again the brain weight by a few percentage. The obtained results make possible to estimate error size in morphometric studies conducted on the formaldehyde-fixed material. It was stated also that the above histological procedure causes more pronounced changes in fetal rat brain and in brain of 1-2-day-old rats. Stabilization of changes caused by histological procedure is achieved between first and sixth months of rat life.
这些研究的目的是详细阐述用于定量研究的材料制备技术程序，并确定形态计量参数的校正系数与组织学程序的依赖关系。此外，还估计了大鼠黑质和纹状体（帕金森病中发生退化的结构）在生命的前半年中的体积变化。使用4℃和20℃的4%和8%甲醛作为固定剂。无论采用何种固定方式，甲醛在最初48小时内都会使脑重量和体积迅速增加，达到新鲜脑重量和体积的52%，随后脑重量缓慢下降，约为1-3%/24小时。20℃的4%甲醛引起的变化最为明显。使用20℃的8%甲醛可获得形态计量研究的最佳条件。用乙醇脱水会使脑体积和重量急剧下降（从新鲜脑重量的32%降至39%）。用甲基苯甲酸酯透明会使脑重量再次增加几个百分点。所得结果使得能够估计在甲醛固定材料上进行的形态计量研究中的误差大小。还指出，上述组织学程序在胎鼠脑和1-2日龄大鼠脑中引起的变化更为明显。组织学程序引起的变化在大鼠生命的第一个月到第六个月之间趋于稳定。
