Sadowski M, Moryś J, Berdel B, Maciejewska B
Department of Anatomy, Medical University, Gdańsk.
Folia Morphol (Warsz). 1995;54(4):219-26.
The influence of fixation and histological procedure on morphometric parameters of human and rat brains were analyzed. We found negative correlation of brain weight with the fixation time both in human and rat material. Human brains fixed for shorter period of time than one year showed large differences in weight. The group of brains fixed for longer than one year was characterized by much smaller dispersion. Moreover, we found that during histological preparation the shrinkage of brain tissue of older human subjects is statistically significantly smaller than younger ones (below 65 years). In order to correct these artifacts easily we propose three simple formulas.
分析了固定和组织学程序对人和大鼠大脑形态计量学参数的影响。我们发现，在人和大鼠材料中，脑重量与固定时间均呈负相关。固定时间短于一年的人脑重量差异较大。固定时间超过一年的脑组其离散度要小得多。此外，我们发现，在组织学制备过程中，老年受试者脑组织的收缩在统计学上显著小于年轻受试者（65岁以下）。为了便于校正这些假象，我们提出了三个简单的公式。
