Freidel Christoph, Kaloyanova Stefka, Peneva Kalina
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128, Mainz, Germany.
Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University Jena, Jena Center of Soft Matter, Lessingstr. 8, 07743, Jena, Germany.
Amino Acids. 2016 Jun;48(6):1357-72. doi: 10.1007/s00726-016-2204-5. Epub 2016 Mar 11.
This review focuses on the various approaches to covalently attach a chromophore to a biomolecule of interest in site-specific manner. Novel methods like inverse electron-demand Diels-Alder reaction, Pictet-Spengler ligation and enzyme tags like SNAP and Halo-tags are critically discussed and compared to established techniques like copper-free click reaction and native chemical ligation. Selected examples in which the tags have been exploited for in vitro or in vivo imaging are reviewed and evaluated.
本综述聚焦于以位点特异性方式将发色团共价连接到目标生物分子上的各种方法。对逆电子需求狄尔斯-阿尔德反应、皮克特-施彭格勒连接等新方法以及SNAP和卤代标签等酶标签进行了批判性讨论，并与无铜点击反应和天然化学连接等成熟技术进行了比较。对利用这些标签进行体外或体内成像的选定实例进行了综述和评估。
